生物型异种骨软骨移植修复关节软骨缺损  

Articular cartilage defect treated with biological osteochondral xenogenic graft transplantation

作　　者：沈洪园[1] 黄华扬[1] 张余[1] 郑小飞[1] 李凭跃[1] 徐国风 王泽锦[1] 王庆[1] 

机构地区：[1]解放军广州军区广州总医院,广东省广州市510010 [2]广东冠昊生物科技有限公司,广东省广州市510663

出　　处：《中国组织工程研究》2013年第44期7729-7732,共4页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技计划项目(2007B031000003)~~

摘　　要：背景:目前应用的各种软骨修复方法均有其优点和不足,修复组织多以纤维软骨为主,缺乏正常透明软骨的组织化学特性和生物力学特性。目的:探讨生物型异种骨软骨移植修复关节软骨损伤的可行性。方法:实验动物普通山羊随机分成两组,实验组供体为猪膝关节,以Smith&Nephew骨软骨移植器械,取直径4.5 mm、长10 mm的圆柱状骨软骨条,应用专利技术对动物骨软骨进行去抗原,对照组供体为羊膝关节骨软骨条,以低温冷冻法保存。分别于股骨滑车及内侧髁负重面的损伤处作骨软骨植入,术后16,32周处死动物,进行大体形态及病理切片观察。结果与结论:大体观察实验组部分软骨缺损移植处有纤维样的组织覆盖;部分移植物原表面软骨发黄,表面与周缘软骨有明显界限;对照组大体及切片镜下观察软骨缺损区基本被移植软骨覆盖,移植边缘可见裂隙。结果可见生物型异种骨软骨移植修复关节软骨损伤的作用与同种异体骨软骨比较有差别,同种异体骨软骨移植修复效果更佳。BACKGROUND： The methods used to repair articular cartilage defects currently have the cons and pros. Fibrocartilages are commonly used to repair tissues, and the fibrocartilage lacks of the tissue biomechanical properties and chemical properties of normal hyaline cartilage. OBJECTIVE： To investigate the feasibility of biological osteochondral xenogenic graft transplantation to repair articular cartilage defects. METHODS： The normal goats were randomly divided into two groups. The donor pig knee joints were the experimental group. Cylindrical osteochondral with the diameter of 4.5 mm and length of 10 mm were collected with the Smith ＆ Nephew osteochondral transplantation device, and the patented technology was used for deantigen. The donor goat knee joint osteochondrals were the control group and preserved with cryopreservation. The lesions on femoral trochlea and weight-bearing surface of medial condyle were selected respectively for osteochondral implantation, and the animals were sacrificed at 16 and 32 weeks after operation for the general and pathological section observation. RESULTS AND CONCLUSION： General observation in the experimental showed that the lesions were covered by fibroid tissue; some cartilage of the grafts turned yellow and there was clear boundary between the surfaceand the peripheral cartilages; the general and section observation under microscope showed that lesions of the contro group were covered by the grafts basically, and cracks could be seen on the edge of the transplant part. The results show that there is difference between effects of biological osteochondral xenogenic graft transplantation and osteochondral allograft transplantation for the repairing of articular cartilage defects, and osteochondral allograft transplantation bas better effect.

关 键 词：器官移植 组织移植 异种异体 同种异体 骨软骨移植 软骨损伤 修复 羊 猪 去抗原处理 省级基金 

分 类 号：R617[医药卫生—外科学]