肝内移植骨髓基质细胞对大鼠肝纤维化损伤修复及其磁共振示踪观察  

Injury repairing and magnetic resonance tracing of transplanted bone marrow stroma! cells in rat liver with hepatic fibrosis

作　　者：范国华[1] 龚建平[1] 沈钧康[1] 张彩元[1] 徐孝秋[1] 徐亮[1] 俞泽阳[1] 张维[1] 吴浩荣[2] 

机构地区：[1]苏州大学附属第二医院影像科,215004 [2]苏州大学附属第二医院普外科,215004

出　　处：《中华医学杂志》2013年第39期3135-3138,共4页National Medical Journal of China

基　　金：江苏省苏州市应用基础研究计划（SYS201041）;苏州大学附属第二医院博士、留学归国人员预研项目（SDFEYBS1107）

摘　　要：目的探讨肝纤维化环境中移植骨髓基质细胞（BMSCs）在肝内迁移分布规律及其MR示踪的可行性。方法超顺磁性氧化铁（SPIO）及5一溴脱氧尿核苷（Brdu）双标记BMSCs经门静脉移植入大鼠肝纤维化模型，分别于移植后2h、3d、7d、2周行MR扫描，分析不同时间点BMSCs在肝内MR信号和病理组织学特点。结果（1）标记的BMSCs经门静脉移植术后MR检查见多发结节状低信号灶随时间向肝内分散。（2）含铁血黄素染色及Brdu免疫组织化学染色阳性细胞：移植术后2h主要分布于肝门部门静脉内，移植术后3d主要分布于门静脉小分支、肝窦内、肝小叶中央静脉周围，移植术后7d、2周主要分布于肝损伤较重区和纤维间隔，部分BMSCs与肝细胞紧密连接，形成规则的肝细胞索。各时间点MRI信号改变与组织学所见一致。结论（1）经门静脉移植BMSCs随时间逐渐向肝内移行，以损伤较重区和纤维间隔分布明显，具有选择性分布特点，对肝损伤具有修复作用；（2）MR信号变化规律在一定程度上反映了移植细胞在肝内的分布、迁移和分化状态。Objective To explore the recurring patterns of migration and distribution of transplanted bone marrow stromal cells （BMSCs） in rat model with hepatic fibrosis and the feasibility of magnetic resonance （MR） tracing. Methods BMSCs labeled with 5-bromodeoxyuridine （Brdu） and superpara-magnetic iron oxide （SPIO） were transplanted into rat model with hepatic fibrosis via portal vein. MR scan was performed at Hour 2, Day 3, Day 7 and Week 2 post-transplantation to analyze the hepatic features of MR signal intensity and pathohistology of BMSCs. Results Multiple hypo-intense lesions appeared in hepatic hilar region at Hour 2 and became smaller with the elapsing time. Hemosiderin and Brdu immunohistochemical stains showed that positive cells were found in portal vein of hepatic porta at Hour 2, small branches of portal vein, sinus hepaticus and around central veins of hepatic lobules at Day 3 and liver parenchyma （esp. in area of lesion） at Day 7 and Week 2. Some of transplanted BMSCs were tightly connected with liver cell to form liver cell cord. The signal intensity changes of MRI corresponded to histological findings at different timepoints. Conclusion The transplanted BMSCs are gradually scattered in whole liver （esp. in lesion area） so that it may help to repair hepatic lesions. And the recurring patterns of MR signal intensity changes reflected the condition of distribution, immigration and differentiation of transplanted cells.

关 键 词：磁共振成像 示踪剂 骨髓基质细胞 

分 类 号：R445.2[医药卫生—影像医学与核医学] R575.2[医药卫生—诊断学]