骨髓浓缩液-PGA支架复合在兔关节软骨修复中对骨髓刺激的作用  

Combination of bone marrow concentrate and PGA scaffolds enhancing bone marrow stimulation in the repair of rabbit articular cartilage

作　　者：王守国[1] 费昊东[1] 季峰[1] 谢跃[1] 李凯[2] 田纪伟[2] 赵庆华[2] 

机构地区：[1]南京医科大学附属淮安市第一人民医院骨科,223300 [2]上海交通大学附属第一人民医院骨科

出　　处：《中华医学杂志》2013年第39期3147-3151,共5页National Medical Journal of China

摘　　要：目的观察骨髓浓缩液-PGA支架复合在兔关节软骨修复中对骨髓刺激的作用。方法30只成年雄性新西兰白兔，麻醉后构建兔的膝关节软骨缺损模型，将所有兔子分为5个治疗组：无治疗组（空白对照）、骨髓刺激（BMS）组、BMS4-PGA支架植入组、BMS＋PGA支架与自体骨髓浓缩液（BMC）复合物植入组和BMS＋PGA支架与骨髓干细胞（BME）复合物植入组。通过软骨大体观、HE染色、甲苯胺蓝染色及免疫组化等方法处理，评估骨髓浓缩技术对骨髓刺激增强作用及兔软骨缺损修复情况。结果BMS后软骨损伤区平均MSC数量与从髂嵴获得后MSC的数量大致相同，（26．3±6．0）MSCs比（27．6±6．5）MSCs。兔软骨缺损区可见明显的新生软骨形成，白色光滑的修复组织填充缺损区，与周围组织整合较好。HE染色见损伤区以非常有序的组织填充事其表面规则，并且与原软骨组织整合较好；甲苯胺蓝染色、Ⅱ型胶原蛋白免疫组化染色较前3组更强、更平滑、更规则，ICRS视觉组织学评分也显著高于前3组，与BME组结果相似。修复效果明显优于前3组。结论骨髓浓缩液-PGA支架复合对骨髓刺激具有增强作用，是一种简易、有效的方法，有望应用于关节软骨的修复。Objective To observe the effects of bone marrow concentrate （ BMC）-PGA scaffolds for bone marrow stimulation enhancement and repairing rabbit articular cartilage. Methods A rabbit model of articular cartilage defect was established for BMC-PGA stent implantation. After 8 weeks, the experimental animals were sacrificed. And the methods of hematoxylin and eosin stain, toluidine blue stain and immunohistochemistry were used to evaluate the effects of bone marrow stimulation enhancement and rabbit cartilage defect repairing. Results Visible new cartilage formation was evident after implantation. As compared with other groups, the repairing effect was better. Conclusion The implantation of BMC-PGA scaffolds is both simple and effective in the repair of articular cartilage.

关 键 词：骨髓移植 刺激剂 软骨形成 组织修复 

分 类 号：R687.4[医药卫生—骨科学]