烟草丛顶病伴随RNA临沧分离物基因组全长序列测定和结构分析  

Complete Nucleotide Sequence and Genome Organization of A Lincang Isolate of Tobacco Bushy Top Disease-associated RNA

作　　者：赵兴能[1,2] 陈海如[1] 莫笑晗[2] 

机构地区：[1]云南农业大学植物保护学院,云南昆明650201 [2]云南省烟草农业科学研究院,云南昆明650031

出　　处：《安徽农业科学》2013年第20期8480-8480,8524,共2页Journal of Anhui Agricultural Sciences

基　　金：云南省科学技术厅项目(2011FB143);云南省烟草公司项目(2011YN36-1)

摘　　要：[目的]对烟草丛顶病伴随RNA(TBTDaRNA)临沧分离物基因组全长序列进行测定,并对其进行结构分析。[方法]应用RT-PCR方法扩增烟草丛顶病伴随RNA临沧分离物的基因组,并进行全长序列的测定,同时对烟草丛顶病伴随RNA临沧分离物和龙陵分离物的基因组进行比较分析。[结果]烟草丛顶病伴随RNA临沧分离物和龙陵分离物的基因组全长序列一致性为95.4%;ORF1a编码产物的氨基酸序列一致性为93.1%;ORF1b编码产物的氨基酸序列一致性为98.3%;3'端非编码区的核苷酸序列一致性为95.5%。[结论]该研究为研究TBTDaRNA的分子变异奠定了基础。[Objeetive] The aim was to sequence the complete nueleotide and analyze the genome organization of a Lineang isolate of Tobacco bushy top disease-associated RNA （TBTDaRNA）. [ Method] The full -length genome of a Lineang isolate of TBTDaRNA was amplified by RT- PCR and sequenced. Comparisons were carried out at the genomes level for TBTDaRNA Lineang isolate and Longling isolate. [ Result] The TBT- DaRNA Lineang isolate and Longling isolate had a 95.4% nueleotide identity in the full - length genomie sequence, and 93.1% and 98.3 % anli- no acid identity between their ORF1 a and ORF1 b, respeetively ; the nueleotide identity between their 3＇untranslated regions was 95.5 %. [ Conclu- sion] The study laid a foundation for the research of molecular variability of TBTDaRNA.

关 键 词：烟草丛顶病伴随RNA 基因组 序列测定 

分 类 号：S572[农业科学—烟草工业]