烟草内生细菌Itb295对黑胫病菌的抑制及其鉴定  

Identification and Inhibition of Tobacco Endophytic Bacteria Strain Itb295 against Black Shank Pathogens

作　　者：杨桃[1] 吴道军[1] 王勇[2] 邢小军[2] 徐莉[1] 马冠华[1] 

机构地区：[1]西南大学植物保护学院,重庆400716 [2]四川省烟草公司凉山州公司,四川西昌615000

出　　处：《安徽农业科学》2013年第20期8525-8528,共4页Journal of Anhui Agricultural Sciences

基　　金：中央高校基本科研业务费专项(XDJK2010C083);四川省烟草专卖局项目(201002007)

摘　　要：[目的]确定分离自健康烟草植株的内生细菌Itb295的分类地位和生防潜能。[方法]通过平板对峙培养法测定菌株Itb295对烟草黑胫病菌的抑制作用,并结合菌株形态、生理生化特性和16S rDNA序列(GenBank登录号JF496206)比对分析确定该菌株的分类地位。[结果]鉴定Itb295菌株为枯草芽孢杆菌Bacillus subtilis。菌株Itb295对黑胫病菌菌丝生长抑制率为34.64%,受抑制的黑胫病菌菌丝膨大、扭曲、分支增多,不能正常生长,抑菌效果的可逆性分析显示受抑制的菌丝在OA培养基上能够正常生长。[结论]为烟草黑胫病的防治及内生细菌Itb295的进一步研究提供了理论依据。[ Objective] The aim was to deter mine taxonomic taxa and potential application of endophytic bacteria Itb295 obtained from healthy tobacco plants. [ Method] The inhibitory ability of Itb295 against Phytophthora nicotianae was investigated with dual-cultured. The Itb295 was i- dentified according to morphological,physiological and biochemical characteristics, and 16S rDNA （GenBank accession JF496206） phylogenetic analysis. [ Result ] Itb295 was identified as Bacillus subtilis. The Itb295 significantly inhibited the mycelial growth of P. nicotianae with average in- hibition rate as 34.64%. The mycelia of P. nicotianae treated with hb295 had a compact branch and showed swollen and distortion. Antibacterial effect of the reversibility analysis showed inhibition of hyphae on excluding medicine tablet could normal growth. [ Conclusion] The research re- suits provide theoretical basis for the control of black shank pathogens and further study on endophytic bacteria Itb295.

关 键 词：内生细菌 烟草黑胫病菌 抑制 鉴定 

分 类 号：S572[农业科学—烟草工业]