HPLC双波长梯度洗脱法同时测定清热灵颗粒中3组分的含量  

Determination of the content of three constituents in Qingreling Granules by Dual Wave-length HPLC with Gradient Elution

作　　者：田雁钰 

机构地区：[1]河南省南阳市食品药品检验所,南阳473008

出　　处：《海峡药学》2013年第10期41-43,共3页Strait Pharmaceutical Journal

摘　　要：目的 建立同时测定清热灵颗粒中黄芩苷、连翘苷和靛玉红含量的HPLC双波长梯度洗脱法.方法 色谱柱：Kromasil C18（250mm×4.6mm,5μm）.以乙腈（A）-0.5%三乙胺水溶液（磷酸调pH至3.05）（B）为流动相梯度洗脱.检测波长λ1=277nm,λ2=289nm,流速：1.0mL·min-1;柱温：25℃.结果 黄芩苷线性范围为1.64～104.96μg·mL-1（r=0.9999）,平均回收率为98.98%,RSD为0.37%（n=6）;连翘苷线性范围为1.62～103.63μg·mL-1（r=0.9999）,平均回收率为98.84%,RSD为0.23%（n=6）;靛玉红线性范围为0.75～48.03μg·mL-1（r=0.9999）,平均回收率为98.34%,RSD为0.68%（n=6）.结论 方法简便可靠,结果重现性好,为控制清热灵颗粒的内在质量提供了科学依据.OBJECTIVE To establish a method for determination the content of baicalin and forsythin and In- dirubin in Qingreling Keli by HPLC. METHODS The Kromasil C is column （250mm×4.6mm,5μm） was used, The mobile phase was acetonitrile （A）-0. 5% triethylamine（ adjust pH to 3.05 with phosphoric acid） （B）. The de- tection wavelength was at λ1= 277nm, k2 = 289nm, the flow rate was 1.0mL·min - 1. The temperatu re of the column at 25℃. RESULTS The linear range for baicalin and forythin and Indirubin was 1.64 - 104. 96μg·mL-1 （ r = 0. 9999） andl. 62 -103.63μg·mL-1（r =0. 9999） and 0. 75 -48. 03μg·mL-1（ r =0. 9999） ,the average recov- ery was 98.98% （ RSD 0. 37%, n = 6） and 98.84% （ RSD 0. 23%, n = 6） and 98.34% （ RSD 0. 68%, n = 6） o CONCLUSION The method is simple, accurate, reliable and can be applied to the quality control of Qingreling Keli.

关 键 词：清热灵颗粒 黄芩苷 连翘苷 靛玉红 HPLC法 

分 类 号：R927.2[医药卫生—药学]