饥饿和切除眼柄对日本囊对虾卵巢发育过程中肝胰腺内生化组分含量的影响  

Influence of starvation and eyestalk ablation on the biochemical composition of hepatopancreas during ovarian development of Marsupenaeus japonicas

作　　者：李飞[1] 吴超[1] 林琼武[1] 王桂忠[1] 李少菁[1] 

机构地区：[1]厦门大学海洋与地球学院,福建厦门361005

出　　处：《应用海洋学学报》2013年第4期524-529,共6页Journal of Applied Oceanography

基　　金：国家高科技发展计划863资助项目(2006AA10A406);福建省科技计划资助项目(2012N0030)

摘　　要：在水温为23.5±1.5℃,pH =7.0 ～8.0,盐度为30 ～32,光照为1 000 Lux左右的条件下,对来自台湾海峡的日本囊对虾亲虾（体质量为83.26±1.47 g/ind.）进行饥饿和切除眼柄的处理,实验为期9d,设置对照组、切除眼柄组、饥饿组和饥饿＋切除眼柄4个组,采用形态解剖称量法和酶学法测定亲虾卵巢发育过程肝胰腺内葡萄糖、胆固醇和甘油三酯含量的变化情况.实验结果表明,繁殖期间亲虾肝胰腺中的葡萄糖、胆固醇和甘油三酯的含量变化随着促熟时间延续呈下降走势,其中切除眼柄组亲虾肝胰腺内葡萄糖含量和胆固醇含量变化最大,分别从（66.72±3.82）× 10-3、（48.70±4.81）×10-3 mg/g下降到（12.13±1.52） ×10-3、（14.50±1.47）×10-3 mg/g,而切除眼柄＋饥饿组甘油三酯含量从（32.00±3.18）×10-1 mg/g下降到（5.92±1.24）×10-1 mg/g,降幅达到81.50％;实验过程3种生化储备物含量水平,切除眼柄组一直低于对照组,切除眼柄＋饥饿组低于饥饿组,而饥饿组远远低于对照组;分析认为繁殖期间亲虾肝胰腺中葡萄糖、胆固醇和甘油三酯的吸收和积累小于转移;切除眼柄能促进亲虾肝胰腺中葡萄糖、胆固醇和甘油三酯通过血淋巴转移到性腺,加快能量的转化效率;性腺发育所需的生化储备物质主要来源于食物,饥饿不利于性腺发育.The morphological anatomy,weighing method and enzymatic method were used to investigate the changes of glucose,cholesterol and triglyceride of hepatopancreaslevels during the ovarian development of Marsupenaeus japonicas （weight 83.26 ± 1.47g） caught from Taiwan Strait.The organisms,starved and eyestalk ablated,cultured inwater temperature 22 ～ 25 ℃,salinity 30 ～ 32 and pH 7.0 ～ 8.0,were divided into 4 groups,i.e.the control group,eyestalk ablation,starvation,starvation with eyestalk ablation.The results showed that glucose,cholesterol and triglyceride of hepatopancreascontentsdecreasedwith the maturation.The greatest change group of glucose content and cholesterol content in hepatopancreas was the eyestalk ablation.There were decreased from（66.72 ± 3.82）× 10-3 mg/g,（48.70 ±4.81） × 10-3mg/g to（12.13 ± 1.52） × 10-3 mg/g,（14.50 ± 1.47） × 10-3mg/g respectively.The triglyceride content in hepatopancreas of starved with eyestalk ablated broodstock was decreased from （32.00 ±3.18） × 10-1 mg/g to（5.92 ± 1.24） × 10-1 mg/g,and the decreasing range was 81.50％.Threebiochemical contents of eyestalk ablation group were lower than the control group.The group of starvationwitheyestalk ablation was lower than the starvation group.The starvation groupwas much lower compared with the control group.It considered that the absorption and accumulation of glucose,cholesterol and triglyceride were slowerthan the transportation during reproductive period.The eyestalk ablation could promote the transfer of glucose,cholesterol and triglyceride to ovary through hemolymph and improve energy conversion efficiency.However,starvation was harmful for ovarian development because biochemical reserve substances,which is from food,aresubstantial for ovarian development.

关 键 词：海洋生物学 日本囊对虾 饥饿 切除眼柄 肝胰腺 生化组分 

分 类 号：Q955[生物学—动物学]