纤维环穿刺法建立兔腰椎间盘退变模型  

Establishing the Rabbit Model of Degenerative Intervertebral Disc by Annulus Puncture

作　　者：杜炎鑫[1] 佟德民[2] 邓晋丰[1] 郭玉海[1] 陈博来[1] 禤翠玲[3] 符路娣[4] 谭滴清[4] 

机构地区：[1]广东省中医院骨一科,广州广东510120 [2]广州医科大学附属第五医院,广州广东510700 [3]广州中医药大学第一附属医院,广州广东510405 [4]广州中医药大学动物实验中心,广州广东510405

出　　处：《南京中医药大学学报》2013年第5期459-461,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：广东省科技计划项目(2009B030801292)

摘　　要：目的建立纤维环穿刺法建立兔椎间盘退变模型。方法 40只新西兰大白兔随机分为牵引组和对照组,手术组(30)只以16G针刺损伤新西兰兔腰椎椎体右前侧纤维环制备椎间盘退变模型,对照组(10只)不予穿刺手术。分别于造模术后4、8、12周通过腰椎侧位X片及组织病理学检查观察椎间隙变化情况及组织病理情况。结果手术组在腰椎侧位X线片上术后4周椎间隙高度逐渐下降,椎间盘高度指数变化率(DHI)2组差异有统计学意义(P<0.05);且随着时间的推移,手术组椎间盘内细胞逐渐减少。结论纤维环穿刺法可缓慢模拟人类损伤后椎间盘退变,操作安全可靠。OBJECTIVE To establish the rabbit model of degenerative intervertebral disc by annulus puncture. METHODS Forty New Zealand white rabbits were randomly divided into 2 groups. Operation group was for annulus fibrosus with a 16- gauge needle damaging the right front side of lumbar vertebrae in 30 New Zealand white rabbits while the rest ten rabbits were used as control group without any annulus puncture. The changes in intervertebral space and pathological conditions were re- spectively examined with lateral spine X-rays and histopathological exam ination on 4, 8 and 12 weeks after operation. RE- SULTS The height of intervertebral space in operation group was gradually decreased and the difference of the rate of change of DHI between the two groups was statistically significant from the fourth week on （P^0.05）. The histological sections dem- onstrated that the cell content in nucleus pulposus in operation group gradually decreased. CONCLUSION The annulus punc- ture may slowly authentically initate human disc degeneration after injury and the modeling method is safe and reliable.

关 键 词：纤维环穿刺 椎间盘退变 动物模型 兔 

分 类 号：R285.5[医药卫生—中药学]