槐果碱对冠脉结扎诱发大鼠急性心肌缺血的影响  

Effects of Sophocarpine of Rats Acute Myocardial Ischemia Induced by Coronary Artery Ligation

作　　者：狄灵[1] 李睿萍[1] 杨成志[1] 刘素蓉[1] 杨海霞[1] 

机构地区：[1]西安交通大学医学院第二附属医院,陕西西安710004

出　　处：《南京中医药大学学报》2013年第5期462-465,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：陕西省科技攻关项目(2010K17-02)

摘　　要：目的通过观察槐果碱对冠状动脉结扎诱发大鼠急性心肌缺血SOD、MDA、心电图ST-T的改变及CX43的影响,探讨槐果碱对心肌缺血的影响,为槐果碱的临床应用提供实验证据。方法实验分为假手术组、模型组、胺碘酮组及不同剂量槐果碱组,连续灌胃7d,采用左冠状动脉结扎术诱发SD大鼠致心肌缺血。分别记录造模前、造模后即刻及造模后24h正常Ⅱ导联心电图,然后股动脉采血,分离血清,测定超氧化物歧化酶(SOD)、丙二醛(MDA)含量变化,并取心脏缺血部位,做病理切片,免疫组化法观察CX43的分布变化。结果①与假手术组比较,模型组血清超氧化物歧化酶(SOD)明显降低(P<0.01)。血清丙二醛(MDA)明显升高(P<0.01)。与模型组相比,胺碘酮、槐果碱中剂量组、槐果碱大剂量组SOD明显升高(P<0.05),胺碘酮、槐果碱大剂量组MDA明显降低(P<0.05)。②与假手术组比较,模型组ST-T在造模后即刻和24h时均明显升高(P<0.01)。与模型组相比,胺碘酮组、槐果碱小剂量组、槐果碱中剂量组、槐果碱大剂量组造模后即刻心电图ST-T明显降低(P<0.01)。③假手术及处理组心肌纤维胞质内CX43表达较模型组多,排列较乱。胺碘酮、槐果碱小剂量组、槐果碱中剂量组、槐果碱大剂量组平均光密度值明显降低(P<0.01)。结论槐果碱能够增加血清超氧化物歧化酶含量,降低丙二醛含量,改善心肌缺血,还可以对抗心肌缺血引起缝隙连接蛋白CX43的分布异常。OBJECTIVE To observe effects of sophocarpine to SOD, MDA and Connexin43 in acute myocardial ischemia rats induced by coronary artery ligation, to explore the mechanism of action of the sophocarpine and to provide laboratory evidence for the application of sophocarpine. METHODS The experimental animals which were divided into sham operation, model group, amiodarone group and different doses sophocarpine groups were administrated for 7 days, and were ligated the left coro- nary artery to induce SD rats myocardial ischemia, II lead electrocardiogram was recorded before, during and after model estab- lishment. Blood from femoral artery was collected and its serum was separated to detect the content of SOD and MDA. The is- chemic part of the rat＇s heart was selected for histological examination. Immunohistochemistry was applied to observe the dis- tribution of CX34.RESULTS ① Compared with the sham group, SOD decreased significantly （P〈0.01）, while MDA was significantly higher （P〈0.01）. Compared with the model group, amiodarone, sophocarpine middle dose group, sophocarpine big dose group SOD was significantly higher （P〈0.05）, amiodarone, sophocarpine high-dose group MDA decreased signifi- cantly （P〈0.05）. CX43 expression within the cytoplasm. ②Compared with the sham group, model group, ST-T immediate- ly after modeling and 24 hours was significantly higher （P〈0.01）. Compared with the model group, amiodarone group sopho- carpine middle dose group, the high dose group of sophocarpine, after modeling instantly ECG ST-T was significantly de- pressed （P〈0.01）, ST-T sophocarpine low-dose group depression （P〈0.05）. ③ Myocardial fibers within the cytoplasm of sham-operated and treated Cx43 expression compared with the model group, arranged chaos, amiodarone group, small-dose group of sophocarpine , middle-dose group of sophocarpine , high-dose group of sophocarpine, the average optical density value decreased significantly （P〈0.O1）.CONCLUSION The sophocarpine can increase

关 键 词：槐果碱 冠脉结扎 急性心肌缺血 

分 类 号：R285.5[医药卫生—中药学]