隔姜灸对胃癌大鼠VIP和IL-10水平的影响  

Effects of Ginger Moxibustion on VIP and IL-10 in Gastric Cancer Rats

作　　者：江薇[1] 喻国华[2] 陈建章[2] 唐翔宇[1] 龚晓军[2] 张宏泉[1] 吴娟[1] 王进[1] 

机构地区：[1]江西中医药高等专科学校医学基础部,江西抚州344000 [2]江西中医药高等专科学校医疗系,江西抚州344000

出　　处：《南京中医药大学学报》2013年第5期466-468,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江西省卫生厅中医药科研基金(2009A036)

摘　　要：目的观察隔姜灸对胃癌大鼠组织血管活性肠肽(VIP)、白介素10(IL-10)的影响。方法采用(N-甲基-N-硝基-N-亚硝基胍)MNNG建立胃癌模型,造模后随机分为VIP组、VIP受体拮抗剂组、模型组、隔姜灸4组,每组10只,另设空白组10只。VIP组和VIP受体拮抗剂组分别每日皮下注射VIP或VIP受体拮抗剂0.1μg/mL,空白组给予生理盐水,隔姜灸组隔姜灸中脘、脾俞、胃俞、足三里、内关等穴位,3周后检测胃癌VIP、IL-10含量的变化,通过免疫组织化学和RT-PCR检测胃腺癌组织中VIP蛋白和mRNA表达情况。结果模型组胃癌组织VIP、IL-10明显增高,隔姜灸组较模型组含量明显降低(P<0.01),与VIP治疗组比较差异也有显著性(P<0.01)。胃癌组织出现VIP免疫反应阳性细胞,相对正常的胃组织中均未见VIP阳性细胞。胃癌组织中均有VIP mRNA表达。VIP治疗组和隔姜灸均能下调VIP mRNA表达,但隔姜灸效果更佳(P<0.01)。结论隔姜灸可能通过降低大鼠胃癌组织中VIP、IL-10含量及抑制VIP mRNA表达而达到治疗胃癌的目的。OBJECTIVE To observe the effect of ginger moxibustion on vasoactive intestinal peptide（VIP） and interleukin-10 （IL-I0） in gastric carmer rats. METHODS The gastric carcinoma models, estab[ished by N-methyl-N-nitro-N-nitrosoguanidine （MNNG）, were randomly divided into 4 groups： VIP group（n= 10, 10 μg/100 mL）, VIP antagonist group（n= 10, 10 μg/100 mL）, model group（n= 10, the normal breeding） and ginger moxibustion group（ginger moxibustion, n= 10, on zhongwan, pishu, weishu, zusanli, neiguan, etc）. Apart from that, blank group（n=10, physiological saline） was set up additionally. Three weeks later, the contents of V1P, IL-10 were tested, and the expression of proteins VIP and mRNA in gastric cancer tis- sues were detected by immunohistrochemistry and RT-PCR methods. RESULTS VIP and IL-10 in model group and were re duced, and the ones in ginger moxibustion group were increased obviously（P〈0.01）, which have significant differences with the VIP group. Gastric carcinoma group have VIP-positive immunoreactive cells, which were not found in normal gastric tis sues. There was VIP mRNA expression in each groups＂ gastric cancer tissue. The expression of mRNA in Ginger moxibustion and VIP groups.were reduced, but ginger moxibustion group was more significantly reduced（P〈0.01）. CONCLUSION Gas- tric cancer rats can be treated with ginger moxibustion by inhibiting the detection of VIP, IL-10 and the expression of VIP mR- NA.

关 键 词：胃癌 隔姜灸 VIP IL-10 

分 类 号：R285.5[医药卫生—中药学]