Chromatographic Analysis of Thiophenes in Calli and Suspension Cultures of Tagets spp.  

作　　者：Hussein S. Taha Hamida A. Osman Mahmoud M.M.A. Youssef Abdel Monem Y. EI-Gindi Hoda H.Ameen Asmahan M.S. Lashein 

机构地区：[1]Department of Plant Biotechnology, National Research Centre, Dokki, Cairo, Egypt [2]Department of Plant Pathology, National Research Centre, Dokki, Cairo, Egypt [3]Department of Zoology & Agricultural Nematology, Faculty of Agriculture, Cairo University, Cairo, Egypt

出　　处：《Journal of Life Sciences》2013年第5期483-490,共8页生命科学（英文版）

摘　　要：The thiophene isomer fractions were investigated by TLC （thin layer chromatography） and GLC （gas liquid chromatography）. The TLC analysis revealed that, 5-（4-acetoxy-l-butynyl）-2,2-bithenyl （BBTOAc, Rf 0.78 I）; 5-（3-buten-l-ynyl）-2,2-bithineyl （BBT, Rf 0.39 II） and 2,2,5,2-oc-terthienyl （∝-T, Rf 0.32 III） were detected in extracts of leaf, stem and root calli or suspension cultures of Tagetes erecta and T. patula as well as leaf cell culture of T. patula which treated with DL-tryptophan under light or dark conditions. The GLC quantitative analysis of thiophenes in subjected revealed that the percentage distribution of the major thiophenes in all the analysed samples were determined as follows： BBT II 〉 ∝ -TllI 〉 BBTOAc I. Another thiophene was determined in relatively small amounts and found to be 5-（3-penten-l-ynyl）-2,2-bithienyl （PBT IV）. Other thiophenes were quantitated in trace amounts with unidentified or unknown chemical structure from the investigated extracts.

关 键 词：l＇hiophenes CALLUS suspension TLC GLC Tagetes erecta T. patula. 

分 类 号：Q[生物学]