糖类化合物亲水作用色谱保留行为评价  

Systematic evaluation of retention behavior of carbohydrates in hydrophilic interaction liquid chromatography

作　　者：傅青[1] 王军 梁图[1] 徐晓勇[1] 金郁[1] 

机构地区：[1]华东理工大学药学院,上海200237 [2]营口港务集团有限公司,辽宁营口115007

出　　处：《色谱》2013年第11期1051-1056,共6页Chinese Journal of Chromatography

基　　金：国家自然科学基金项目(21005028);华东理工大学基本科研业务费探索研究基金(WY1214070)

摘　　要：以糖类化合物为研究对象,系统评价了其在亲水模式下的色谱保留行为。分别考察了流动相、固定相和缓冲盐等对糖类化合物保留的影响,建立了糖类化合物在亲水模式下的保留方程。结果表明,糖类化合物随着流动相中乙腈比例的降低,保留时间减小;随着缓冲盐浓度的增加,保留时间增加;同时,糖类化合物的保留行为还会受到有机溶剂种类和固定相类型的影响;其保留行为可使用顶替吸附-液相相互作用模型定量描述。将该模型进一步用于实际样品中糖类化合物保留行为的预测,获得了较好的实验结果,预测保留时间与实测保留时间的相对误差小于0.3%。对糖类化合物亲水模式下的保留行为进行了系统的评价和定量描述,该研究结果将有助于糖类化合物亲水作用色谱分离方法的发展。A systematic evaluation of retention behavior of carbohydrates in hydrophilic interaction liquid chromatography （HILIC） was performed.The influences of mobile phase,stationary phase and buffer salt on the retention of carbohydrates were investigated.According to the results,the retention time of carbohydrates decreased as the proportion of acetonitrile in mobile phase decreased.Increased time of carbohydrates was observed as the concentration of buffer salt in mobile phase increased.The retention behavior of carbohydrates was also affected by organic solvent and HILIC stationary phase.Furthermore,an appropriate retention equation was used in HILIC mode.The retention equation lnk =a ＋ blnCB ＋ cCB could quantitatively describe the retention factors of carbohydrates of plant origin with good accuracy：the relative error of the predicted time to actual time was less than 0.3%.The evaluation results could provide guidance for carbohydrates to optimize the experimental conditions in HILIC method development especially for carbohydrate separation.

关 键 词：亲水作用色谱 保留行为 保留方程 糖类化合物 

分 类 号：O658[理学—分析化学]