异丙酚对呼吸机所致肺损伤大鼠肺组织TGF-β1/Smad2信号通路的影响  

Effects of propofol on transforming growth factor （TGF）-β1-Smad2 signal pathway in rats with ventilator-induced lung injury

作　　者：高巨 葛亚丽 周罗晶 林舜艳 

机构地区：[1]苏北人民医院(扬州大学临床医学院)麻醉科,江苏扬州225001 [2]科教处

出　　处：《中国急救医学》2013年第11期1032-1035,I0003,共5页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金（81171838）

摘　　要：目的 评价不同时点异丙酚对呼吸机所致肺损伤和组织转化生长因子（TGF）-β1-Smad2信号通路的影响.方法 健康雄性Wistar大鼠50只,体质量260～300 g,采用随机数字表法,随机分为五组（每组10只）：常规潮气量通气组（A组,VT=8 mL/kg）、大潮气量通气组（B组,VT =30 mL/kg）、异丙酚不同时点给药组（C～E组）.C组、D组和E组分别于给予大潮气量通气前1h、给予大潮气量通气前即刻和给予大潮气量通气后1h时静脉注射异丙酚5 mg/kg,随后以10 mg/（kg·h）速率输注至机械通气4h结束.收集肺组织标本,测定肺泡灌洗液（BALF）蛋白、肺通透指数（LPI）及肺组织湿/干质量比（W/D）;测定TGF-β1 mRNa和Smad2表达.结果 与A组比较,B～E组W/D、LPI增加,TGF-β1 mRNa和Smad2表达上调（P〈0.05）;与B组比较,C组、D组W/D、LPI降低,TGF-β1 mRNa和Smad2表达下调（P〈0.05）.结论 异丙酚早期给药可减轻大鼠呼吸机所致肺损伤,其机制与抑制TGF-31/Smad2通路激活有关.Objective To investigate the effects of propofol treatments on ventilator - induced lung injury and the expression of transforming growth factor （TGF） - β1 - Smad2 signal pathway of rats. Methods Fifty male Wistar rats （260 -300 g, 7 -8 weeks old） were randomly divided into 5 groups： standard ventilation group （group A, VT = 8 mL/kg）, high -tidal volume ventilation group（ group B, received high- tidal volume ventilation VT = 30 mL/kg）, propofol treated groups （group C, D and E, received propofol 5 mg/kg 1 h before, immediately after and 1 h after high - tidal volume ventilation administration, respectively ）, and then the treated groups were followed by infusion of propofol at 10 mg/（ kg·h）until 4 h after high - tidal volume ventilation. Then the animals were sacrificed, and the lung tissues were immediately removed for calculation of wet/dry lung weight ratio （W/D） and lung permeability index （LPI）. Lung pathologic changes were observed under light microscope, and the expression of TGF - β1 mRNA and Smad2 protein in lung tissue was determined. Results The four experiment groups （B -E） all showed significant increase in W/D, LPI and the expression of TGF -β1 mRNA and Smad2 protein when compared with tile control group（ P 〈0.05 ）. When compared with group B, both groups C and D showed obviously attenuated increase in W/D, LPI and expression of TGF - β1 mRNA and Smad2 （P 〈 0.05）. Conclusion Early propofol administration may attenuate ventilator - induced lung injury, which may be related to the inhibition of TGF - β1 - smad2 signal pathway.

关 键 词：异丙酚 呼吸 人工 成人呼吸窘迫综合征(ARDS) 转化生长因子β1(TGF-β1) SMAD2 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]