大鼠氯胺酮静脉自身给药成瘾模型的建立  

ESTABLISHMENF OF INTRAVENOUS KETAMINE SELF-ADMINISTRATION MODEL IN RATS

作　　者：黄坤玉[1] 黄娴妮[1] 杨澍均[1] 孙艳[1] 吕秀依[1] 马宝苗[2] 刘昱[1] 

机构地区：[1]宁波大学医学院,宁波315211 [2]江汉大学武汉生物医学研究院,武汉430056

出　　处：《中国药物依赖性杂志》2013年第5期337-341,共5页Chinese Journal of Drug Dependence

基　　金：国家自然科学基金(81000573);浙江省自然科学基金(Y2091155);浙江省人力资源和社会保障厅"钱江人才"

摘　　要：目的:建立大鼠氯胺酮静脉自身给药成瘾模型。方法:成年♂SD大鼠左侧颈外静脉插管手术后,训练氯胺酮静脉自身给药,剂量为0.5 mg·kg-1,每天4 h。随后,进行5个不同剂量(0.125、0.25、0.5、0.75和1 mg·kg-1)的累进频率(progressive ratio,PR)测试。最后,进行连续10 d的自身给药行为维持,维持剂量为0.5 mg·kg-1,每天2 h。结果:90%大鼠经过7 d的训练,出现稳定的氯胺酮静脉自身给药行为;PR测试呈现明显的剂量效应;维持期内大多数大鼠仍能保持稳定的自身给药行为,2 h平均给药58次。结论:通过对大鼠自身给药行为训练期、PR测试期和维持期等阶段结果的观察与分析,说明大鼠氯胺酮自身给药模型的成功建立和维持。To establish the animal model of ketamine self - administration in rats. Me ： Following cannulated surgery in the left external jugular vein, adult male SD rats were trained to self - administer ketamine at a dose of 0. 5 mg . kg-1 for 4 hours every day. Subsequently, the animals were tested on the progressive ratio（PR） procedure under five different ketamine doses（0. 125,0. 25, 0. 5,0. 75 and 1 mg . kg-1）. Finally,rats were maintained on fixed ratio（FR） procedure under the dose of 0. 5 mg . kg-1 for consecutive 10 days. Results： 90% of the rats acquired ketamine self - administration during 7 days of training. A significant dose - response effect was observed under the procedure of PR. A majority of the animals exhibited stable self - administration behavior on FR procedure during the maintaining phase. Conclusion： Ketamine self - administration model was successfully established in the current study.

关 键 词：氯胺酮 静脉自身给药 成瘾 动物模型 

分 类 号：R971[医药卫生—药品]