麝香保心丸对心力衰竭大鼠肾脏α_1及β肾上腺素受体各亚型表达的影响  

Effect of Shexiangbaoxin pills on expression of renal α_1-adrenergic receptor and β-adrenergic receptor subtypes in rats with heart failure

作　　者：刘飞[1] 李艳芳[1] 曹芳芳[1] 张玲姬[1] 白雪源[2] 吕杨[2] 

机构地区：[1]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所心内科,北京100029 [2]解放军总医院肾内科

出　　处：《心肺血管病杂志》2013年第5期637-640,共4页Journal of Cardiovascular and Pulmonary Diseases

基　　金：中国中西医结合学会科研基金(2008-01)

摘　　要：目的:探讨麝香保心丸对心力衰竭大鼠肾脏α1-肾上腺素受体(α1-adrenergic receptorα1-AR)和β-肾上腺素受体(β-adrenergic receptorβ-AR)各亚型表达水平的影响。方法:选择雄性Wistar大鼠80只,随机分为正常对照组(10只)、假手术组(10只),其余60只制作心肌梗死后心力衰竭模型,模型成功35只并将其随机分为心力衰竭组(9只)、阳性药物对照组(8只)、麝香保心丸低剂量组(9只)和麝香保心丸高剂量组(9只)。取大鼠肾脏皮质,采用Western Blot分别测定各组大鼠α1-AR和β-AR各亚型蛋白表达水平。结果:假手术组与正常对照组α1A-AR和β1-AR表达水平比较,差异无统计学意义(P>0.05);与假手术组比较,心力衰竭组大鼠α1A-AR、β1-AR表达水平明显下调,差异有统计学意义(P<0.05);与心力衰竭组比较,阳性药物对照组、低剂量组、高剂量组大鼠α1A-AR、β1-AR表达水平明显上调,差异有统计学意义(P<0.05);与低剂量组比较,阳性药物对照组、高剂量组大鼠α1A-AR表达上调更为显著(P<0.05)。各组大鼠α1B-AR、α1D-AR和β2-AR表达水平比较,差异无统计学意义(P>0.05)。结论:麝香保心丸缓解了心力衰竭大鼠肾脏α1-AR和β-AR各亚型表达水平的下调,对恢复肾脏功能、改善心力衰竭起到了有益的作用。同时,与小剂量麝香保心丸相比,高剂量疗效更为显著。Objective：To explore the effect of shexiangbaoxin pills（SXBXP） on expression of renal ctI - adrenergic receptor （Ctl-AR） and B adrenergic receptor （B-AR） subtypes in rats with heart failure. Meth- ods： Eighty male wistar rats were randomly divided into normal control group （n = 10 ） and sham operation group （n = 10）. The heart failure model was established successfully in 35 of the rest 60 male wistar rats. The 35 heart failure model rats were randomly divided into four groups： heart failure model group（ n = 9） , positive medicine control group（ n = 8 ）, low dose of SXBXP group（ n = 9） and high dose of SXBXP group（ n = 9）. The protein levels of ctI -AR and B-AR subtypes of renal cortex were measured by western blot in each group. Re- suits： Compared with normal control group, expression of CtlA-AR and B-AR showed no significant difference in sham operation group（P 〉 0.05 ）, compared with sham operation group, expression of OtIA-AR and B-AR was decreased significantly in heart failure model group （P 〈 0.05 ）. Compared with heart failure model group, expression of cqA-AR and ~x-AR was increased significantly in group positive medicine control group, low dose of SXBXP group and high dose of SXBXP group（P 〈 0.05 ）. Compared with low dose of SXBXP group, expres-sion of otlA-AR was increased significantly in group positive medicine control group and high dose of SXBXP group（ P 〈 0.05 ）. Expression of c^ls-AR,ctlD-AR and ~2-AR showed no difference in each group（ P 〉 0.05 ）. Conclusion： SXBXP lessen the downregulation of expression of renal oh-AR and 13-AR subtypes in rats with heart failure, and it is also effective to recover renal function and improve heart failure, especially high dose of SXBXP.

关 键 词：肾上腺素受体 麝香保心丸 肾脏 心力衰竭 

分 类 号：R54[医药卫生—心血管疾病]