温阳化瘀法对体外培养的内异症内膜细胞VEGF分泌的影响  

Influence of Wenyang Huayu Method on VEGF Secretion in Endometrial Cells of Patients with Endometriosis Cultured in Vitro

作　　者：刘立群[1] 李理[1] 黄艳辉[2] 李银广[1] 

机构地区：[1]中山大学附属第一医院,广东广州510700 [2]广州中医药大学第二临床医学院,广东广州510120

出　　处：《中医药信息》2013年第5期28-31,共4页Information on Traditional Chinese Medicine

摘　　要：目的:观察温阳化瘀法对体外培养的子宫内膜异位症患者在位、异位内膜细胞血管内皮生长因子(VEGF)分泌的干预作用。方法:将成功体外培养的15例内异症患者在位、异位内膜细胞(实验组)及10例非内异症患者在位内膜细胞(对照组)进行分组,实验组随机分为5组,即温阳化瘀法高、中、低剂量组、孕三烯酮组、空白对照组。采用酶联免疫吸附试验(ELISA)法检测各组上清液中VEGF的水平。结果:体外培养的子宫内膜异位症异位、在位内膜细胞上清液中VEGF水平较非内异症的高,采用温阳化瘀法干预后可以降低VEGF水平(P<0.05)。结论:子宫内膜异位症发生与VEGF表达失调有关,温阳化瘀法可以降低VEGF水平。Objective：To observe the interventive effect of Wenyang Huayu method on VEGF secretion in eutopic endometrium and ectopic endometrium of patients with endometriosis cultured in vitro.Methods：The eutopic endometrium and ectopic endometrium of 15 patients with endometriosis and the eutopic endometrium of 10 non endometrium controls were cultured in vitro and then were randomly divided into six groups：the high dosage group with Wenyang Huayu method,the medium one and the low one,Gestrinong group,normal control group,and non endometrium control group.VEGF level in the supernatant in each group was detected with ELISA.Results：VEGF level in the supernatant of eutopic endometrium and ectopic endometrium of patients with endometriosis cultured in vitro went up.Wenyang Huayu drug could reduce the expression of VEGF.Conclusion：The occurrence of endometriosis is associated with abnormal expression of VEGF.Wenyang Huayu drug can result in down-regulation of VEGF.

关 键 词：子宫内膜异位症 VEGF 温阳化瘀法 

分 类 号：R271.9[医药卫生—中西医结合]