补阳还五汤对AD大鼠海马区β-APP mRNA、IL-1β mRNA、IL-6mRNA、TNF-α mRNA表达影响的研究  

Influences of Buyang Huanwu Decoction on Expression of β- APP mRNA,IL- 1β mRNA,IL- 6 mRNA and TNF- α mRNA of Hippocampus of Alzheimer Disease Rats

作　　者：严妍[1] 姜波[1] 杨茂[2] 周忠光[1] 

机构地区：[1]黑龙江中医药大学药物安全性评价中心,黑龙江哈尔滨150040 [2]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001

出　　处：《中医药信息》2013年第5期41-43,共3页Information on Traditional Chinese Medicine

基　　金：黑龙江省自然科学基金项目(D200912)

摘　　要：目的:采用分子杂交技术观察补阳还五汤对Aβ1-40所致老年痴呆(AD)大鼠海马区β-APP mRNA、IL-1βmRNA、IL-6 mRNA及TNF-αmRNA表达的影响,进一步探讨该组方治疗AD的机制,为其临床应用提供实验依据。方法:70只Wistar雄性大鼠随机分为正常组、假手术组、模型组、补阳还五汤组、补气组、活血组和脑复康组,每10只。采用注射Aβ1-40的方法制备AD模型,分子杂交技术检测大鼠海马区β-APP mRNA、IL-1βmRNA、IL-6 mRNA、TNF-αmRNA的表达。结果与结论:补阳还五汤能够明显降低AD大鼠海马区β-APP mRNA、IL-1βmRNA、IL-6mRNA和TNF-αmRNA的表达,从而改善大脑记忆障碍,达到防治AD的作用。In this study,molecular hybridization technique is used to observe the effect of Buyang Huanwu decoction on the expression of β-APP mRNA,IL-1β mRNA,IL-6 mRNA and TNF-α mRNA of hippocampus of Alzheimer Disease（AD）rats induced by Aβ1-40,to further explore the mechanism of the treatment of AD,and to provide experimental evidence for the clinical application.The results show that Buyang Huanwu decoction can significantly reduce the β-APP mRNA,IL-1β mRNA,IL-6 mRNA and TNF-α mRNA expression in AD hippocampus,thus to improve memory disorders and to play a role in the prevention of AD.

关 键 词：补阳还五汤 AD Β-APP MRNA IL-1β MRNA IL-6 MRNA TNF-ΑMRNA 分子杂交技术 

分 类 号：R285.5[医药卫生—中药学]