裂片石莼水提物清除ABTS和DPPH自由基的光谱学研究  

Spectrometric investigation of DPPH and ABTS free radical-scavenging of Ulva lacyuca L. aqueous extracts

作　　者：钟钰[1,2] 陈填烽[3] 郑文杰[3] 杨宇峰[1,2] 

机构地区：[1]暨南大学水生生物中心,广州510632 [2]水体富营养化与赤潮防治广东省教育厅重点实验室,广州510632 [3]暨南大学化学系,广州510632

出　　处：《生态科学》2013年第4期447-452,共6页Ecological Science

基　　金：国家科技支撑计划课题(2012BAC07B05;2012BAD18B01);暨南大学重点培育项目(21612106)资助

摘　　要：研究了大型海藻裂片石莼和富硒裂片石莼水提物清除2,2’-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)(ABTS)及1,1-二苯基-2-三硝基苯肼(DPPH)自由基能力及其光谱学特征。分光光度计法测定,水提物ABTS自由基体系测定波长为734 nm,体系稳定时间为6 min;DPPH自由基体系测定波长为515 nm,体系稳定时间为30 min。结果表明,裂片石莼水提物具有良好的抗氧化活性,能有效、快速地抑制溶液中ABTS和DPPH自由基。在优化选择的反应体系中,裂片石莼、富硒裂片石莼和标准抗氧化剂VC对ABTS自由基的半数抑制浓度(IC50)分别为76.40μg/ml、54.11μg/ml和60.69μg/ml,说明富硒裂片石莼水提取的总抗氧化活性明显优于VC,富硒培养可以显著提高裂片石莼的抗氧化活性。裂片石莼、高硒裂片石莼和标准抗氧化剂VC对DPPH自由基的IC50值分别为123.29μg/ml、76.825μg/ml和24.787μg/ml。综合上述,富硒培养裂片石莼水提物具有优良的抗氧化活性,对水溶性自由基的抑制率明显高于脂溶性自由基,有广阔的开发前景。The antioxidant activities of Ulva lacyuca L. aqueous extracts against DPPH and ABTS free radicals were evaluated using spectrometric methods. The results showed that the detection wavelength and stable time for ABTS system were 734 nm and 6 min respectively,while those for DPPH system were 515 nm and 30 min,respectively. The extracts effectively and rapidly removed ABTS and DPPH free radicals,and showed a dose- and time-dependent manner,indicating the potent antioxidant activities under both hydrophilic and hydrophobic conditions. Under the optimized systems,the IC50values for Se-PPC,PPC and VC against ABTS free radical were 76.40,54.11 and 60.69 μg/ml,respectively. Se-PPC exhibited significantly higher activities than the VC. For DPPH free radicals,the IC50values of Se-PPC,PPC and VC were 123.29,76.83 and 24.79 μg/ml,respectively. Our results suggest selenium-enrichment cultured U. lacyuca L. has potential value of applications to be functional food with antioxidation.

关 键 词：石莼 硒 抗氧化活性 ABTS DPPH 

分 类 号：Q945.78[生物学—植物学]