甘草黄酮对大强度耐力运动大鼠肾脏组织Ca^(2+)、Mg^(2+)-ATPase及Bax,Bc1-2蛋白表达的影响  

Effect of High-Intensity Endurance Exercise on Ca^(2+),Mg^(2+)-ATPase and Bax,Bcl-2 Protein Expression With Glycyrrhiza Flavonoids in rat Nephridial Tissue

作　　者：王东旭[1] 陈艳艳[2] 

机构地区：[1]塔里木大学体育工作部,新疆阿拉尔843300 [2]西北大学体育部,陕西西安710069

出　　处：《四川体育科学》2013年第5期30-34,共5页Sichuan Sports Science

摘　　要：目的:探讨甘草黄酮对大强度耐力运动大鼠肾脏组织Ca2+、Mg2+-ATPase及Bax、Bcl-2表达的影响。方法:选取SD雄性健康大鼠24只,随机分为安静组、大强度运动组和运动加药组;采用跑台训练6周后取材,应用试剂盒和免疫组织化学法测检测各组大鼠肾脏组织Ca2+、Mg2+-TPase活性及Bax和Bcl-2表达的变化。结果:与安静对照组相比,大强度运动组和运动加药组肾脏组织Ca2+、Mg2+-TPase活性均呈非常显著性下降(P<0.01);其中运动加药组Ca2+、Mg2+-TPase活性均较大强度运动组具有非常显著差异性提高(P<0.01);大强度耐力运动组和运动加药组大鼠细胞凋亡指数AI均有不同程度增加;大强度运动组Bax、Bcl-2蛋白表达(MOD)均呈非常显著差异性(P<0.01),运动加药组Bax蛋白表达(MOD)均呈非常显著差异性(P<0.01);运动加药组Bcl-2蛋白表达(MOD)与大强度运动组具有非常显著差异性(P<0.01);大强度运动组和运动加药组Bax/Bcl-2比值分布呈不同程度的显著差异性(P<0.05,P<0.01)。结论:甘草黄酮能够增强对大强度运动大鼠抗氧化能力,降低肾脏组织自由基的产生,抑制组织细胞的凋亡。Objective To explore Glycyrrhiza Elavonoids on the rat nephridial tissue of Ca2＋, Mg2＋-ATPase and Bax, Bcl-2 protein expression with high-intensity endurance exercise. Methods The twenty-four healthy male rats were randomly divided into quiet groups, high-intensity exercise group and exercise plus Glycyrrhiza Elavonoids group, After 6 weeks of treadmill training, Using the box of reagent and immunity histochemistry examined the changing of Ca2＋, Mg2＋-ATPase and Bax, Bcl-2 protein expression on each groups . Results Compared with the quiet groups, the activity of Ca2＋, Mg2＋-ATPase both had significant droped （P〈0.01）, and the groups of plus drog had very difference increased than high-intendity exerxise groups （P〈0.01）; High-intensity endurance exercise group and exercise dosing rats AI apoptosis index increased in varying degrees;high-intensity exercise group （MOD） were very significant difference（P〈0.01）, exercise plus drug group Bac protein expression （MOD）were very significant difference （P〈0.01）; Exercise plus drug group Bcl-2 protein expression（MOD） with the high-intersity exercise group had significant difference（P〈0.01）, High-intensity exercise group and exercise plus drug group Bax/Bcl-2 ratio of distribution is significantly difference degrees of difference（P〈0.05,P〈0.01）.

关 键 词：甘草黄酮 大强度耐力训练 肾脏 ATP BAX Bcl-2 

分 类 号：G804.2[文化科学—运动人体科学]