Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish  

作　　者：WANG Yang ZHOU Li LI Zhi LI WenHua GUI JianFang 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology, Chinese Academy of Sciences

出　　处：《Science China(Life Sciences)》2013年第11期975-984,共10页中国科学（生命科学英文版）

基　　金：supported by grants from the National Basic Research Program of China(2010CB126300);the National High Technology Research and Development Program of China(2012AA092201-3);Ministry of Agriculture of China within the special Fund for Agro-scientific Research in the Public Interest(200903045);the National Natural Science Foundation of China(30971414)

摘　　要：Apolipoprotein C1 （Apocl） is associated with lipoprotein metabolism, but its physiological role during embryogenesis is largely unknown. We reveal a new function of Apoclb, a transcript isoform of Apocl, in epiboly during zebrafish gastrulation. Apoclb is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos. It displays a ra- dial gradient with high levels in the interior layer and low levels in the superficial layer. Knockdown of Apoclb by injecting antisense morpholino （MO） caused the epiboly arrest in deep cells. Moreover, we show that the radial intercalation and the ra- dial gradient distribution of E-cadherin are disrupted both in Apoclb knockdown and overexpressed embryos. Therefore, Apoc 1 b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner.Apolipoprotein C1(Apoc1)is associated with lipoprotein metabolism,but its physiological role during embryogenesis is largely unknown.We reveal a new function of Apoc1b,a transcript isoform of Apoc1,in epiboly during zebrafish gastrulation.Apoc1b is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos.It displays a radial gradient with high levels in the interior layer and low levels in the superficial layer.Knockdown of Apoc1b by injecting antisense morpholino(MO)caused the epiboly arrest in deep cells.Moreover,we show that the radial intercalation and the radial gradient distribution of E-cadherin are disrupted both in Apoc1b knockdown and overexpressed embryos.Therefore,Apoc1b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner.

关 键 词：EPIBOLY apolipoprotein C1 E-cadherin radial intercalation 

分 类 号：Q954.4[生物学—动物学]