溶液中稳定分散的介孔二氧化硅纳米球的制备及其生物毒性研究  

Synthesis of mesoporous silica nanospheres and study of their biotoxicities

作　　者：方天[1] 田迎[2] 王建东[3] 滕兆刚[2] 孙晶[2] 恽时锋[1] 

机构地区：[1]南京军区南京总医院比较医学科,南京医学硕士210002 [2]南京军区南京总医院医学影像科,南京210002 [3]南京军区南京总医院病理科,南京210002

出　　处：《医学研究生学报》2013年第10期1014-1018,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金(30970813)

摘　　要：目的介孔纳米材料有望成为一种新型的药物载体应用于药物领域，文中制备在溶液中能稳定分散的介孔二氧化硅纳米球（MesoporousSilicaNanospheres，MSNs）并探讨其生物毒性。方法以十六烷基三甲基溴化铵为模板剂，采用溶胶凝胶法制备MSNs，利用透射电子显微镜、X射线衍射、氮气吸附-脱附仪检测MSNs的物理化学性质；检测MSNs对293T细胞（人胚肾细胞）增殖的影响；观察BALB／c小鼠尾静脉注射20mg／kg和40ms／kg剂量MSNs后肝、脾、肾、心、脑和肺的病理学变化。结果制备的MSNs在溶液中可稳定分散，平均粒径为100nm，具有高度有序的二维六方结构。体外实验结果：50～400μg／ml范围内，MSNs对293T细胞成活率没有影响（P〉0．05）；体内实验结果：40mg／kgMSNs导致BALB／c小鼠肝和肺出现病理变化。结论在溶液中稳定分散的MSNs对293T细胞没有毒性；长时间尾静脉注射高剂量MSNs对小鼠肝和肺有一定程度的损伤，通过控制MSNs的使用剂量与使用周期可以避免对动物组织器官的毒副作用。Objective Mesoporous silica nanospheres （MSNs） promise to be a new drug carrier. This paper aimed to synthesize MSNs with stable dispersibility in prepared solution and explore their biotoxicities. Methods MSNs were prepared via sol-gel technology with cetytrimethylananonium bromide （CTAB） as the template, and then characterized using transmission electron microscopy （TEM） ima- ges, X-ray diffraction （XRD） patterns and nitrogen sorption isotherms. The effect of MSNs on the motility of 293T cells was detected, and the histopathological changes in the liver, spleen, kidney, heart, brain and lungs after intravenous injection were observed. Results The prepared MSNs were stably dispersed in the solution. Within 50 -4430 μg/ml, MSNs exhibited no biological toxicities on 293T cells in vitro （P 〉 0. 05）, but induced pathological injuries in the liver and lungs of the BALB/c mice after intravenous injected at 40 mg/kg. Conclusion Stably dispersed MSNs in the solution had no biotoxicity on 293T cells, but long-term intravenous injection of high-dose MSNs could damage the liver and lungs of mice. However, the toxicity of MSNs can be avoided by controlling their concentration and administration time.

关 键 词：介孔二氧化硅纳米球 细胞毒性 组织病理学 

分 类 号：R944.2[医药卫生—药剂学]