miR-21通过PTEN/AKT通路抑制白血病细胞K562的迁移和增殖  

miR-21 inhibits migration and proliferation of leukemic K562 cells via the PTEN/AKT pathway

作　　者：吴共发[1] 黄绮亭[1] 曾宇婷[1] 林俊汕[1] 叶梓莹[1] 胡纯婷[2] 

机构地区：[1]广东省增城市人民医院(中山大学附属博济医院)病理科,增城511300 [2]南方医科大学基础医学院病理学系,广州510515

出　　处：《医学研究生学报》2013年第10期1037-1040,共4页Journal of Medical Postgraduates

摘　　要：目的 miR-21在多种肿瘤中的研究已有大量文献报道,但其对白血病细胞迁移和增殖的影响及其可能机制尚未完全阐明,文中旨在探讨抑制miR-21对白血病K562细胞迁移和增殖能力的影响及可能的作用机制。方法将miR-21 inhibitor用LipofectamineTM2000转染K562细胞,荧光定量PCR检测miR-21的表达水平;Transwell迁移实验和Cell Counting Kit-8(CCK-8)法测细胞迁移和增殖变化;Western blot检测PTEN、AKT、磷酸化AKT(p-AKT)蛋白表达变化。结果抑制miR-21表达后K562细胞的迁移和增殖能力均受到明显抑制;抑制miR-21能明显增强PTEN蛋白和减低p-AKT蛋白表达水平,对AKT蛋白水平无明显影响。结论抑制miR-21表达可能通过调控PTEN/AKT通路抑制白血病K562细胞迁移和增殖。Objective There are numerous reports about miR-21 in tumors, but its role in the migration and proliferation of leukemia and possibly involved mechanism is not yet completely understood. This study aimed to investigate the effects of micro RNA- 21 （miR-21） on cell migration and proliferation in leukemic K562 cells and further elucidate its possible mechanism. Methods miR-21 inhibitor was transfected into K562 cells by LipofectamineTM2000. MiR-21 expression was detected by quantitative RT-PCR. Cell migration and proliferation were studied by Transwell migration assay and Cell Counting Kit-8 assay （CCK-8）. The expressions of PTEN, AKT and p-AKT proteins were determined by Western blot. Results Suppressed miR-21 significantly inhibited the migration and proliferation of leukemic K562 cells. Inhibition of miR-21 did not affect the expression of total AKT, whereas the phosphorylated AKT level was markedly down-regnlated and the PTEN protein expression up-regnlated. Conclusion Suppression of miR-21 could inhibit the migration and proliferation of leukemic K562 cells via the PTEN/AKT pathway.

关 键 词：MIR-21 白血病 PTEN AKT 

分 类 号：R557[医药卫生—血液循环系统疾病]