二烯丙基三硫醚对幽门螺杆菌生物膜的杀菌作用  

Antimicrobial effect of diallyl trisulfide on Helicobacter pylori biofilms

作　　者：刘伟[1] 于晗[1] 李玉瑭[1] 刘旭静[1] 李雯[1] 单玉群[1] 孙允东[1] 周亚滨[1] 

机构地区：[1]山东大学医学院病原生物学研究所山东省感染与免疫重点实验室,济南250012

出　　处：《山东大学学报（医学版）》2013年第10期49-53,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(ZR2009CM073)

摘　　要：目的探讨二烯丙基三硫醚(DATS)对幽门螺杆菌生物膜的杀菌作用,进一步研究生物膜耐药的机制。方法微量肉汤法测定药物的最小抑菌浓度,平板培养法构建幽门螺杆菌生物膜的体外模型,XTT法检测药物处理后浮游状态与生物膜状态下的幽门螺杆菌活性,激光共聚焦显微镜观察生物膜深层的细菌状态以及活性变化。结果克拉霉素与DATS对浮游状态的幽门螺杆菌均有良好的杀菌作用;使用不同浓度克拉霉素处理后的幽门螺杆菌生物膜活性明显低于对照组;DATS处理后的幽门螺杆菌生物膜活性下降明显,与对照组和克拉霉素组有统计学差异。结论 DATS对浮游状态和生物膜状态下的幽门螺杆菌均具有良好的杀菌活性,可以为耐药菌临床治疗提供参考。Objective To investigate the antimicrobial effects of diallyl trisulfide （DATS） on Helicobacter pylori （ H. pylori） biofilms and to explore the drug-resistance mechanism of biofilms. Methods The minimal inhibitory con- centration of selected antimicrobials were measured by broth microdilution assay; The biofilm models in vitro were es- tablished on agar plate; The cell activity of planktonic and sessile H. pylori treated with selected antimicrobials was measured with XTT reduction assay; The morphology and activity changes of H. pylori in deep biofilm were observed by confocal laser scanning microscopy. Results Both clarithromycin and DATS elicited strong antimicrobial activity a- gainst planktonic and sessile H. pylori; Different concentrations of clarithromycin significantly reduced the cell activity of H. pylori biofilm; The cell activity of the DATS treated group was significantly lower than that of the control group and the clarithromycin treated group. It had statistical differences. Conclusion DATS elicits strong antimicrobial activ- ity against both planktonic and sessile H. pylori. It may act as an alternative chemotherapeutic agent for multidrug-resist- ant bacterial strains.

关 键 词：幽门螺杆菌 生物膜 二烯丙基三硫醚 抗菌活性 

分 类 号：R378.3[医药卫生—病原生物学]