银屑病患者成纤维细胞生长因子1、丝裂原活化蛋白激酶1、CD34的表达及意义  

Expressions of fibroblast growth factor-1, mitogen activated protein kinase-1 and CD34 in the lesions of patients with psoriasis vulgaris and their significance

作　　者：焦健[1] 张春红[2] 吕世峰 

机构地区：[1]山东大学齐鲁医院皮肤科, 济南250012 [2]山东大学第二医院皮肤科 [3]山东省日照市东港区人民医院

出　　处：《中华皮肤科杂志》2013年第11期784-787,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金青年科学基金（81202700,H2709）;山东省自然科学基金青年基金（ZR2010HQ032）;山东中医药科技发展计划项目（2011-201）;山东省科技攻关项目（2010G0020231）

摘　　要：目的探讨寻常性银屑病皮损成纤维细胞生长因子1（FGF-1）、丝裂原活化蛋白激酶1（MAPK-1）、CD34与临床病理的相关性。方法收集30例寻常性银屑病患者的皮损，记录患者的-般情况和临床表现。用组织芯片技术和免疫组化法检测银屑病皮损中FGF-1、MAPK-1、CD34蛋白的表达，反转录PCR（RT—PCR）法检测银屑病皮损中FGF-1mRNA的表达，统计分析患者的性别、年龄、临床分期、PASI评分、血管新生、表皮异常增生与FGF-1蛋白表达水平的相关性。结果与对照皮肤相比，寻常性银屑病皮损中FGF-1蛋白和mRNA表达均明显上调，差异有统计学意义（P〈0．05）。CD34和MAPK-1在银屑病皮损中均表达上调，且与FGF-1表达呈正相关。FGF-1表达上调与患者的性别和年龄无关，与临床分期和PASI评分相关，其中进行期FGF-1表达上调人数远多于静止期和低评分患者的人数，差异有统计学意义（P〈0．05）。结论FGF-1可能参与了寻常性银屑病表皮异常增殖和真皮乳头层血管异常增生，表达上调与疾病进展和皮损加重相关。Objective To estimate the relationship of fibroblast growth factor-1 （FGF-1）, mitogen activated protein kinase-1 （MAPK-1） and CD34 expressions with clinicopathological findings in lesions of psoriasis vulgaris. Methods Skin specimens were collected from 30 patients with psoriasis vulgaris, whose general information and clinical findings were recorded. The protein expressions of FGF-1, MAPK-! and CD34 in these specimens were detected by tissue chip technology and immunohistochemistry, and the mRNA expression of FGF-1 by reverse transcription-PCR. The correlations of FGF-1 protein expression with patients＇ gender, age, clinical stage, psoriasis area and severity index （PASI） score, angiogenesis and abnormal epidermal proliferation were statistically analyzed. Results The protein and mRNA expressions of FGF-1 were significantly higher in the lesions than in the control skin （both P 〈 0.05）. The protein expressions of CD34 and MAPK-1 were significantly increased, and positively associated with the protein expression of FGF-1 in psoriatic lesions. The up-regulation of FGF-1 protein was unrelated to the age or gender of patients, but associated with clinical stage and PASI score. The proportion of patients with increased FGF-1 expression was significantly larger in patients at active stage and with high PSAI score than in those at quiescent stage and with low PSAI score （P 〈 0.05）. Conclusions In psoriasis vulgaris, FGF-1 may participate in the abnormal epidermal proliferation and vascular dysplasia in dermal papilla, and the unrezulation of FGF-1 appears to be associated with the progression of disease and aggravation of lesions.

关 键 词：银屑病 成纤维细胞生长因子1 丝裂原活化蛋白激酶1 抗原 CD34 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]