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机构地区:[1]河北农业大学动物科技学院,河北保定071000
出 处:《中国家禽》2013年第20期28-31,共4页China Poultry
基 金:国家蛋鸡产业技术体系建设专项资金(CARS-41-K18)
摘 要:本试验旨在研究外源性瘦素对蛋鸡血液生化指标及行为学相关指标的影响。选用260日龄体况相似的海兰褐商品蛋鸡20只,随机分成4组,每组5只。试验I组为对照组,只注射瘦素稀释液(0.75%BSA—PBS),试验Ⅱ、Ⅲ、Ⅳ组为瘦素注射组,注射水平分别为50μg/kg·d、200μg/kg·d、400txg/kg·d。结果表明:血糖浓度,试验Ⅲ、Ⅳ组与对照组相比显著下降(P〈0.05);采食量,试验Ⅳ组与对照组相比显著下降(P〈0.05)。饮水量,试验Ⅱ、Ⅲ、Ⅳ与对照组相比显著下降(P〈0.05),各试验组之间采食频率与饮水频率差异极显著(P〈0.01);各试验组之间梳羽、伸展、转身等行为差异不显著(P〉0.05);争斗发生次数,试验Ⅱ、Ⅲ、Ⅳ组与对照组相比下降极显著(P〈0.01)。综上所述,外源性瘦素注射水平为200-400μg/kg·d能够显著降低蛋鸡血糖含量,抑制蛋鸡争斗行为,降低蛋鸡摄食量,在行为上表现为采食、饮水频率降低,.The purpose of the experiment is to research the effect of exogenous leptin on blood indexes and behaviors of layers. 20 commercial Hyline layers of 260 days of age with similar body condition were randomly divided into 4 groups with 5 replications per group. As control group, group I were treated with leptin diluent. Group Ⅱ, Ⅲ and IV were treated with leptin at dose of 50 μg/kg d, 200 μg/kg.d and 400 μg/kg-d. The results showed that blood glucose level of test m, Ivdeclined significantly compared with control group (P〈0.05). Compared with control group,the food intake of group IV and the water intake of Group Ⅱ , Ⅲ and IV decreased significantly (P〈0.05). Difference of feeding frequency and drinking frequeney in each group were extremely prominent (P〈0.01).There was no difference in feather combing,stretching,turn-back in all groups (P〉0.05),but the fight of test groups decreased compared with control group (P〈0.01). In conclusion, the injection dose of exogenous leptin in 200-400 μg/kg· d could reduce the level of blood glueose and the occurrence of fight significantly, the depression of feeding in behaviors were characterized bylower frequency.
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