鞣花酸对HEK-293细胞DNA损伤作用  

Ellagic acid provokes DNA damage in human HEK-293 cells

作　　者：王东[1] 姜丽萍[1] 刘晓芳[1] 耿成燕[1] 仲来福[1] 陈敏[1] 

机构地区：[1]大连医科大学卫生学教研室,辽宁大连116044

出　　处：《中国公共卫生》2013年第11期1630-1632,共3页Chinese Journal of Public Health

摘　　要：目的探讨鞣花酸诱导HEK-293细胞DNA损伤与氧化应激的关系。方法鞣花酸处理HEK-293细胞后,运用彗星试验检测细胞DNA损伤情况;免疫组化方法检测细胞内8-羟基脱氧鸟苷(8-OHdG)表达水平;紫外分光光度计测定细胞内活性氧(ROS)及还原性谷胱甘肽(GSH)水平。结果 120μmol/L鞣花酸作用1 h能够引起细胞彗星样拖尾,彗星尾部DNA%、尾长、尾矩分别为(12.34±4.21)%、(20.76±3.59)μm和(3.16±0.74),均高于对照组;细胞内ROS含量明显增加(P<0.01),GSH水平下降(P<0.05),60、120μmol/L鞣花酸作用3 h后均引起细胞内8-OHdG表达水平升高(P<0.01)。结论鞣花酸处理HEK-293细胞后使细胞氧化与抗氧化系统失衡,氧化应激引起的氧化性损伤可能是鞣花酸致细胞DNA损伤的重要原因之一。Objective To investigate ellagic acid（EA）-induced DNA damage and the roles of oxidative stress in the damage in HEK-293 cells.Methods The single cell gel electrophoresis assay（SCGE） was used to detect DNA damage.To elucidate the possible mechanism of DNA damage caused by EA in HEK-293 cells,we used fluorescence spectrophotometer to monitor the levels of reactive oxygen species（ROS） and glutathione（GSH）.8-hydroxyderoxy-guanosine（8-OHdG） was also measured with immunocytochemistry staining analysis.Results The EA at 120 μmol/L,for one hour caused DNA migration in the SCGE with significant increases in tail DNA%（12.34±4.21μm）,tail length（20.76±3.59 μm）,and tail moment（3.16±0.74）.The increased level of intracellular ROS（P〈0.01） and the decreased level of intracellular GSH（P〈0.05） were observed.EA at dose of 60 μmol/L-120 μmol/L for 3 hours caused a significant oxidative damage through increased 8-OHdG formation in HEK-293 cells.Conclusion EA could break the balance of oxidative system and anti-oxidative system and the oxidative stress may be an important mechanism in EA-induced DNA damage in human HEK-239 cells.

关 键 词：鞣花酸 DNA损伤 氧化应激 

分 类 号：R994.2[医药卫生—毒理学]