贵州省1959—2006年伤寒菌株PFGE分型及分布特征  

Pulsed field gel electrophoresis based molecular typing and epidemiologic analysis of 214 strains of Salmonella typhi isolated in Guizhou province from 1959 to 2006

作　　者：田克诚[1] 周晖[1] 阚飙[2] 刁保卫[2] 唐光鹏[1] 姚光海[1] 游旅[1] 蔡星和[1] 

机构地区：[1]贵州省疾病预防控制中心,贵阳550004 [2]中国疾病预防控制中心

出　　处：《中国公共卫生》2013年第11期1647-1650,共4页Chinese Journal of Public Health

摘　　要：目的了解贵州省1959-2006年214株伤寒沙门菌的分子流行病学特征,为追踪传染来源、查找传播途径提供线索。方法用脉冲电场凝胶电泳(PFGE)方对贵州省214株分离自不同时间、不同地区的伤寒沙门菌进行PFGE带型分析及其流行病学特征研究。结果根据细菌染色体DNA的XbaI酶切图谱,将贵州省214株伤寒沙门氏菌分成85个带型,除CN0004、CN0016和CN0018等3个带型在国内已有发现外,其余82个带型在国内为首次发现;85个带型中菌株数在5株以上的有13个带型,占15.3%;菌株数2株以上的有12个带型,占14.1%;菌株数为1株的有60个带型,占70.6%。结论伤寒沙门菌PFGE带型复杂,存在明显的基因多态性,是造成贵州省伤寒流行时间长,流行范围广的主要原因。Objective To examine molecular epidemiologic characteristics and similarity of 214 strains of Salmonella typhi（S.typhi） isolated in Guizhou province from 1959 to 2006,and to provide clues for source tracking of infection and finding the route of transmission.Methods Pulsed field gel electrophoresis（PFGE） was applied to type 214 strains of Salmonella isolates.Results According to the restriction map of Xba I,the 214 strains of S.typhi strains were classified in to 85 PFGE types.And 82 types,except for type CN0004,CN0016 and CN0018,were found in China for the first time.Among the PFGE types,there were 13 types with more than 5 strains accounting for 5.1%,12 types with more than 2 strains accounting for 15.31%,and 60 types with more than 1 strains accounting for 70.6%.Conclusion The PFGE types of S.typhi isolated in Guizhou province were complex,with obvious gene polymorphism,which may be the main reason to cause widely and long time typhoid epidemic in Guizhou province.

关 键 词：伤寒沙门菌 脉冲电场凝胶电泳(PFGE) 细菌带型 流行病学特征 

分 类 号：R181.2[医药卫生—流行病学]