规模化制备小牛胸腺DNA的工艺研究  

Research on Large-Scale Preparation of Calf Thymus DNA

作　　者：徐志鹏[1] 杨依顺[1] 邱蔚然 周长林[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009 [2]南通秋之友生物科技有限公司,江苏南通226236

出　　处：《药物生物技术》2013年第5期435-438,共4页Pharmaceutical Biotechnology

基　　金：南通秋之友生物科技有限公司江苏省张友尚院士工作站;江苏省核酸药物工程技术研究中心和江苏省研究生工作站;并由江苏省教育厅2010年高校科研成果产业化推进项目资助(JH10-12)

摘　　要：脱氧核糖核酸(DNA)作为生物遗传物质的基础,具有多种生理功能,广泛用于医药、畜牧业、食品等领域。因此,规模化制备DNA具有良好的经济效益和社会效益。该研究以小牛胸腺为实验材料,对DNA的提取方法和规模化制备工艺进行了研究。在传统的盐溶法及十二烷基硫酸钠(SDS)法基础上,通过2次调节提取液的pH值,进一步提高DNA纯度。采用紫外分光光度法和琼脂糖凝胶电泳法对DNA规模化提取过程中的产物纯度及回收率的变化进行检测。结果表明,规模化提取小牛胸腺30 kg,可得到DNA 549 g,回收率达到1.83%,A260/A28 0值为1.93,纯度为92.3%。该方法工艺简单,适用于规模化工业制备DNA。As the basis of organism genetic material, deoxyribonucleic acid （DNA） has a variety of physical functions in medicine, food, animal husbandry and other fields. Therefore, the large-scale preparation of DNA has good economic and social benefits. To obtain DNA from the calf thymus, DNA extraction methods and large-scale preparation process were studied. The protein was removed by adjusting the pH value twice to improve on the purity of DNA on the basis of the SDS method and salting in method. The quality and purity of DNA during the extraction process were tested by ultraviolet spectroscopy and agarose gel electrophoresis. The results showed that the yield of DNA from 30 kg calf thymus is 549 g, and the recovery is up to 1.83%. Furthermore, the value of A26o/A2so is 1.93 and the purity of DNA is 92.3%. This method is suitable for large-scale preparation of DNA.

关 键 词：小牛胸腺 脱氧核糖核酸 SDS法 规模化制备 纯度 回收率 

分 类 号：Q5[生物学—生物化学]