藏药莪达夏对大鼠心肌缺血再灌注NO和NOS的影响  

Influence of Oxytropis Falcate Bunge on NO and NOS of Myocardial Ischemia-reperfusion Injury in Rats

作　　者：张得钧[1,2] 杜恒[1] 刘明成[2] 李福安[1] 芦殿香[1] 马建滨[3] 

机构地区：[1]青海大学医学院,西宁810001 [2]青海大学生态环境工程学院,西宁810016 [3]青海师范大学生命与地理学院,西宁810008

出　　处：《中国现代应用药学》2013年第10期1054-1058,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：青海省科技厅项目(2011-Z-704);教育部"春晖计划"科研项目(Z2011021)

摘　　要：目的研究藏药莪达夏水提物对大鼠心肌缺血再灌注后,心肌组织中NO含量、一氧化氮合酶(NOS)、诱导性一氧化氮合酶(iNOS)活性的影响,从而探讨其对心肌缺血再灌注损伤保护作用的分子机制。方法采用结扎大鼠冠脉左前降支的方法造成心肌缺血再灌注模型,测定再灌注40 min后心肌组织中NO含量以及NOS、iNOS的活性。结果心肌缺血再灌注模型组心肌组织中NOS、iNOS活性和NO含量水平明显高于正常对照组。莪达夏水提物高、中、低剂量组心肌组织中NOS、iNOS活性水平以及NO含量水平均低于模型组。结论藏药莪达夏能够通过降低NOS、iNOS活性和NO含量水平,从而对大鼠缺血再灌注心肌损伤产生保护作用。ABSTRACT： OBJECTIVE To study the influence of Oxytropisfalcate Bunge on the content of NO, and the activities of NOS and iNOS in myocardial ischemia and reperfushon injury rats to explore its possible protective mechanism in vivo. METHODS The model of myocardial ischemia reperfusion injury in rats was employed by ligating the artery of left anterior descending. After the following reperfusion for 40 minutes, the concentration of NO and the enzymatic activities of NOS, iNOS in rats＇ myocardial tissue were tested. RESULTS The results showed that the enzymatic activities ofNOS, iNOS and the concentration of NO of myocardial tissue in ischemia-reperfusion model group were much higher than that of normal control group. The enzymatic activity of NOS, iNOS and the concentration of NO of myocardial tissue in test groups were lower than that of model group. CONCLUSION Oxytropis falcate Bunge plays protection effects during acute myocardial ischemia-reperfusion injury in rats in vivo by decreasing the enzymatic activity of NOS, iNOS and the concentration of NO.

关 键 词：莪达夏 心肌缺血再灌注 一氧化氮 一氧化氮合酶 

分 类 号：R965.2[医药卫生—药理学]