益智多糖含量测定  

Determination of Polysaccharides Content in Alpinia Oxyphylla

作　　者：赵祥升[1] 董娜[2] 冯锦东[1] 杨美华[1,3] 

机构地区：[1]中国医学科学院药用植物研究所海南分所,海南万宁571533 [2]河南科技学院生命科技学院,河南新乡453003 [3]中国医学科学院药用植物研究所,北京100193

出　　处：《中国现代应用药学》2013年第10期1070-1074,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：中央公益性基金项目(2011HNB04);海南省中药现代化专项项目(2010ZY011)

摘　　要：目的研究益智中多糖含量的测定方法。方法水提醇沉法提取粗多糖,Sevage法除蛋白,H2O2脱色,流水透析和冷冻干燥制备多糖。1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生,HPLC测定其单糖组成。以益智精制多糖测得多糖对葡萄糖的换算因子,苯酚-硫酸法测定益智中多糖的含量。结果益智多糖由葡萄糖、鼠李糖、半乳糖、甘露糖和阿拉伯糖5种单糖组成。益智多糖含量为12.91%,RSD=4.72%(n=5),平均加样回收率为99.1%,RSD为4.57%(n=5)。结论该法简便,快捷,可用于益智多糖的含量测定。OBJECTIVE To establish a method for content determination of polysaccharides in Alpinia oxyphylla. METHODS The crude polysaccharides were extracted by hot water and precipitated with ethanol. The purified polysaccharide was obtained by removing protein and colors with Sevage and H202, repectively, then run water dialysis and frozen-drying. The monosaccharide composition was determined by HPLC after precolum-derivatization with 1-phenyl-3-methyl-5-pyrazolone （PMP）. The conversion coefficient ofAlpinia oxyphylla polysaccharides to glucose was obtained by refined polysaccharides, and then the content of crude polysaccharides in Alpinia oxyphylla was determined by sulfuric acid-phenol method. RESULTS The monosaceharide compositions in polysaccharides from Alpinia oxyphylla were Glu, Rha, Gal, Man, and Ara. The content of polysaccharides was 12.91%（RSD=4.72%, n=5）, and the average recovery was 99.1% with RSD of 4.57%（n=5）. CONCLUSION The method is sample, and can be used for determination of the content of polysaccharides from Alpinia oxyphylla.

关 键 词：益智 多糖 单糖 含量测定 

分 类 号：R917.101[医药卫生—药物分析学]