玉兰酸性转化酶基因的克隆与序列分析  

Gene Cloning and Sequence Analysis of Acid Invertase Gene in Magnolia

作　　者：曹春燕[1] 朱广慧[1] 黄蕊[1] 

机构地区：[1]苏州农业职业技术学院园艺科技学院,江苏苏州215008

出　　处：《北方园艺》2013年第21期120-122,共3页Northern Horticulture

基　　金：江苏省"十一五"农业科技攻关资助项目(BE2007309);江苏省农业三项工程资助项目(SX(2008)043)

摘　　要：以玉兰花瓣RNA为模板,根据GenBank中其它植物转化酶基因的保守序列设计了1对PCR扩增引物,进行玉兰酸性转化酶基因克隆,并对其进行序列分析和氨基酸序列同源性比对。结果表明:该试验克隆获得1条约为500bp的条带;经过序列分析,其长为515bp,属于开放阅读框的一部分,不含内含子,共编码171个氨基酸;该序列及其所编码的氨基酸与其它植物转化酶基因均具有较高的同源性;进化分析显示该基因属于酸性转化酶一类,定位于液泡中。Taking RNA extracted from the petals of Magnolia as template,a pair of PCR primers were designed according to conservative sequence of acid invertase genes in other plants reported in GeM3ank, and acid invertase gene in Magnolia was cloned and its sequence was analyzed. The results showed that a 500 bp fragment was amplified. Sequence analysis showed that the fragment was 515 bp and encoded 171 amino acids. Homology analysis showed that nucleotide and amino acids sequence all shared high similarities with invertase genes cloned from other plants. Phylogenetie analysis revealed that this gene belonged to a class of acid invertase and located in the vacuole.

关 键 词：玉兰 转化酶 克隆 序列分析 

分 类 号：S685.15[农业科学—观赏园艺]