地胆草及其混淆品的显微与薄层鉴别  

Identification of Elephantopi Herba and Its Adulterants by LM /SEM Observation and TLC Scanning

作　　者：曹晖 李药兰[2] 叶文才[2] 李泳雪 毕培曦[4] 邵鹏柱[4] 

机构地区：[1]国家中药现代化工程技术研究中心,广东珠海519020 [2]暨南大学中药与天然药物研究所,广东广州510632 [3]广东省食品药品检验所,广东广州510180 [4]香港中文大学

出　　处：《中药材》2013年第8期1253-1257,共5页Journal of Chinese Medicinal Materials

基　　金：2015年版中国药典科研项目(Z4)

摘　　要：目的:为地胆草及其混淆品的鉴别提供依据。方法:采用光镜(LM)、电镜扫描(SEM)和薄层层析(TLC)法鉴别菊科地胆草属植物(地胆草、白花地胆草)和假地胆草属植物(假地胆草)以及商品药材及其混淆品,获得清晰可靠的显微图谱与薄层指纹图谱。结果:根据细胞测定数据、SEM特征和TLC指纹,证实福建、台湾、香港、澳门地胆草商品药材基原为菊科植物地胆草Elephantopus scaber L.。结论:该方法准确可靠,可用于地胆草及其混淆品的鉴别。Objective：To provide identification basis for distinguishing Elephantopi Herba and its adulterants. Methods：Light mi- croseopy（LM）, scanning electron microscopy（SEM） and thin layer chromatography scanning（TLCS） methods were used to identify Didancao（ Elephantopus scaber） and its adulterants Baihuadidancao （ Elepluzntopus mollis ） and Jiadidancao （ Pseudelephantopus spica- tus）. Results：Based on the microscopic features and TLC profiles, the commercial Didancao samples retailed in China's Mainland, Taiwan, Hang Kong and Macao markets were identified as Elephantopus seaber. Conclusion ： These methods are accurate and reliable, which can be used for identification of Elephantopi Herba and its adulterants.

关 键 词：地胆草 TLC 电镜扫描 鉴定 

分 类 号：R282.5[医药卫生—中药学]