超临界CO_2萃取法与水蒸气蒸馏法提取凤尾草挥发油化学成分的比较  

Comparison of the Chemical Components of Essential Oil Extracted by Supercritical CO_2 Fluid and Steam Distillation from Pteris multifida

作　　者：陈锋[1] 陈欢[2] 李舒婕[1] 林敬明[1] 王兆玉[2] 

机构地区：[1]南方医科大学珠江医院药剂科,广东广州510282 [2]广东药学院生命科学与生物制药学院广东省生物技术候选药物研究重点实验室,广东广州510006

出　　处：《中药材》2013年第8期1270-1274,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(31201236);广东科技厅科技引导项目计划(2011B031700002);广州科信局应用基础研究专项(12C32121594);广东省中医药局科研基金(20121124)

摘　　要：目的:考察超临界CO2萃取法(SFE-CO2法)和水蒸气蒸馏法(SD法)提取的凤尾草挥发油化学成分差别。方法:采用SFE-CO2法和SD法提取凤尾草中的挥发油,用气相色谱-质谱联用仪(GC-MS)分析鉴定其化学成分,用面积归一化法测定各化合物的相对含量。结果:从SFE-CO2法提取的挥发油中鉴定出27种成分,从SD法提取的挥发油中鉴定出45种成分,两者共有成分11种。结论:两种方法提取得到的挥发油化学成分及相对含量有明显差异。Objective ：To compare the chemical components of essential oil extracted by supereritieal CO2 fluid extraction （SFE- CO2 ） and steam distillation extraction（SD） from Pteris multifida. Methods：The essential oil of Pteris multifida was extracted by SFE- CO2 and SD. The chemical components of essential oil were separated and analyzed by GC-MS. Their relative contents were determined by normalization of peak area. Results ： Twenty - seven compounds in the essential oil extracted by SFE-CO2 and 45 compounds in the essential oil extracted by SD were identified respectively. There were 11 common components. Conclusion：The chemical components of essential oil extracted by SEE-CO2 are different from that extracted by SD.

关 键 词：凤尾草 挥发油 超临界CO2萃取法 水蒸气蒸馏法 GC-MS 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]