复方贞芩肺宁胶囊药材的提取工艺及制剂中齐墩果酸的含量测定研究  

Determination of Oleanolic Acid in Fufang Zhenqin Feining Capsules by RP-HPLC

作　　者：容露萍[1] 陈奕伸[2] 廖华卫[3] 万正兰[2] 

机构地区：[1]广州市胸科医院,广东广州510095 [2]中山大学附属第五医院,广东珠海519000 [3]广东药学院中药学院,广东广州510006

出　　处：《中药材》2013年第8期1340-1343,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:优选复方贞芩肺宁胶囊中药材的提取工艺并建立以高效液相色谱法测定复方贞芩肺宁胶囊中齐墩果酸含量的方法。方法:以正交试验设计法优选药材的提取工艺。以Kromasil C18(250 mm×4.6 mm,5μm)为色谱柱,流动相为甲醇-0.3%三乙胺溶液(80∶20),检测波长为210 nm,流速为1.0 mL/min,柱温为30℃,进样量为25μL。结果:按照8倍量70%乙醇,提取3次,每次0.5 h的工艺进行提取。齐墩果酸在59.68~596.8μg/mL范围内进样量与峰面积呈良好的线性关系(r=0.9997),平均回收率为99.79%,RSD为0.5%。结论:该提取工艺合理可行,含量测定方法简便、准确、专属性强,可用于该制剂的含量测定。Objective ： To investigate the optimal technology of extraction of fufang zhenqin feining capsules and establish a method for determination its oleanolic acid. Methods：Orthogonal test was employed for selecting the optimum extraction technology by the index of the content of oleanolic acid in extraction. HPLC was performed on a C l8 （250 mm × 4.6 mm, 5 μm） column using methanol- 0. 3 % triethylamine solution（ 80： 20） as the mobile phase at a flow rate of 1 ml/min. The detection wavelength was set at 210 nm. The column temperature was 30℃ and the injection size was 25 p^L. Resuhs：The optimum extraction technology was as follows：the eoncer- tration of alcohol was 70% , the ratio of solid/material was 8： 1, extracting 3 times with half an hour for each time. The linear range of oleanolie acid was 59. 68 - 596. 8 μg/ml （r = 0. 9997 ） , the average recovery was 99. 79% （ RSD = 0. 5% ）. Conclusion：The considera- ble extraction rate of active components in the drugs is achieved by applying the selected technology, and the simple method is fit for production. The established HPLC method is simple, accurate, special and can be used for the quality control of fufang zhenqin reining capsules.

关 键 词：正交试验法 复方贞芩肺宁胶囊 高效液相色谱法 齐墩果酸 

分 类 号：R286[医药卫生—中药学] R284[医药卫生—中医学]