基于硼酸亲和作用的pH指示剂比色法用于测定糖和核苷类物质  

A colorimetric method by using pH indicators based on the boronic acid affinity interaction for quantity determination of saccharide or nucleoside

作　　者：赵艳艳[1] 王立恒[2] 彭金咏[1] 马骁驰[1] 齐艳[1] 韩旭[1] 尹连红[1] 许丽娜[1] 许有威[1] 梁志忠[1] 

机构地区：[1]大连医科大学药学院,大连116044 [2]大连市第二人民医院康复中心,大连116011

出　　处：《分析试验室》2013年第11期28-30,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21105007);大连市科技项目(2012E15SF145)资助

摘　　要：建立了一种基于硼酸亲和作用的pH指示剂比色法，并应用于糖和核苷类样品的含量测定。选取苯硼酸为硼酸试剂，以糖类和核苷为测试对象，考察并比较不同波长下采用甲基橙和百里酚兰指示剂时溶液浓度与吸光度变化值之间的线性关系。结果表明，糖类物质浓度在10—300mmol／L范围内，核苷类物质浓度在2～36mmol／L范围内，采用百里酚兰指示剂于600nm下测定，线性关系较好。验证实验结果表明方法具有较好的稳定性，准确度和精密度。A colorimetric method by using pH indicators based on the boronic acid affinity interaction was developed, and was applied to quantitative determination of saccharide or nucleoside. Phenyl boronie acid was selected as the boronic acid reagent. Taking saecharide and nueleoside as the test samples, the linear relationship between the absorbance change value （ logI/Io） and the content of test sample was investigated using the pH indicators （methyl orange and thymol blue） under different wavelengths. The result indicated that the linear relationship of absorbance change value （logI/lo） and contents of saccharide （10 ~ 300 mmol/L） or nucleoside （2 - 36 mmool/L） was good at the wavelength of 600 nm with thymol blue as a pH indicator. Moreover, the stability, precision and accuracy were good.

关 键 词：比色法 pH指示剂 糖和核苷 含量测定 

分 类 号：O657.3[理学—分析化学]