毛细管胶束电动色谱法同时测定化妆品中6种对羟基苯甲酸酯类防腐剂  

Simultaneous determination of six parabens in cosmetic products by micellar electrokinetic capillary chromatography

作　　者：沈娟[1] 张海燕[1] 储成顶[1] 任玲玲[1] 梁春梅[1] 

机构地区：[1]安徽医科大学公共卫生学院,合肥230601

出　　处：《分析试验室》2013年第11期88-92,共5页Chinese Journal of Analysis Laboratory

基　　金：安徽医科大学校科研基金项目(2008kj20)资助

摘　　要：建立了同时分离测定化妆品中6种对羟基苯甲酸酯类防腐剂的毛细管胶束电动色谱方法。以未涂层熔融石英毛细管柱(47.5 cm×50μm i.d.,leff=39.5 cm)为分离柱,15 mmol/L Tris-H3PO4(pH 7.0)+10 mmol/L丁二酸二异辛酯磺酸钠(AOT)+体积分数10%乙腈为电泳运行液,10 cm高度差进样,10 s,20kV恒压分离,255 nm波长检测。在质量浓度1.0~200 mg/L范围内,6种组分峰面积与相应质量浓度间均具有良好的线性关系(r≥0.9995)。当信噪比(S/N)为2时,各组分的检出限为1.1~1.7 mg/kg。样品加标回收率为84.2%~122.3%。A micellar electrokinetic capillary chromatographic method was established for simple and simultaneous determination of six parabens most often included in cosmetic products, such as methylparaben （MP）, ethylparaben （EP）, propylparaben （PP）, isopropylparaben （iso-PP）, butylparaben （BP） and isobutylparaben （iso-BP）. Electrophoretic separation was performed on an uncoated capillary （47. 5 cm ×50 μm i. d, left = 39. 5 cm） at ambient temperature （15 ＋1^（2） with a mixed 15 mmol/L Tris - H3PO4 buffer （pH 7.0） containing 10 mmol/L dioctyl sodium sulfosuccinate （AOT） and 10% （V/V） acetonitrile. The other optimum experimental conditions were as follows： siphoning injection of 10 s at 10 cm height difference, applied voltage of 20 kV and detection at 255 nm. The curves of peak response versus concentration for six parabens were linear from 1.0 to 200 mg/L with good correlation coefficients （r^0. 9995）. The detection limits of six parabens were found to be 1.1 - 1.7 mg/kg based on the signal-to-noise ratio of 2. The recoveries were between 84. 2% and 122. 3%.

关 键 词：毛细管胶束电动色谱 对羟基苯甲酸酯 化妆品 

分 类 号：O657.8[理学—分析化学]