光谱法研究核固红-Cu^(2+)-阿莫西林体系及其分析应用  

Spectroscopic study of nuclear fast red-copper (Ⅱ)-amoxicillin system and its analytical application

作　　者：马玲[1] 吴呈珂[1] 彭金娜[1] 冯素玲[1] 

机构地区：[1]河南师范大学化学化工学院,新乡453007

出　　处：《分析试验室》2013年第11期112-115,共4页Chinese Journal of Analysis Laboratory

摘　　要：利用阿莫西林与Cu2+络合导致Cu2+对核固红的荧光猝灭程度减弱的特性,建立了荧光增强分析法检测阿莫西林含量的新方法。讨论了体系的紫外-可见吸收光谱、荧光光谱以及酸度、试剂浓度、反应温度、反应时间和离子强度等因素对体系荧光强度的影响。在最佳实验条件下,荧光强度的增强程度与阿莫西林质量浓度呈线性关系,线性范围为0.40~15.0μg/mL,检出限为0.29μg/mL。考察了一些金属离子和药物赋型物对体系的影响。将方法用于市售阿莫西林胶囊分析,回收率在100.4%~103.4%之间。Amoxicillin can complex with Cu^2＋ , which results in weakening the fluorescence quenching effect of Cu^2＋ to nuclear fast red. Based on that, a new fluorescence method for quantitative determination of amoxicillin has been developed. In this study, the UV - Vis absorption and fluorescence spectra have been investigated. The effects of pH, reaction temperature and time, reagent concentration and ionic strength to the system have been studied. Under the optimum conditions, the enhancement of fluorescence intensity was proportional to the concentration of amoxicillin over the range of 0. 4 - 15 μg/mL with the detection limit of 0. 29 μg/mL. The influence of some metal ions and excipients was also examined. Some amoxicillin capsules have been tested by this method with the recoveries of 100. 4% - 103.4%.

关 键 词：阿莫西林 核固红 CU^2+ 荧光法 

分 类 号：O657.3[理学—分析化学]