Bacillus methylotrophicus菌株产抗菌蛋白性质及对人参锈腐病菌抑菌作用  

Characterization and Inhibition Effects of Antagonistic Protein from Bacillus methylotrophicus on Cylindrocarpon destructans

作　　者：姜云[1] 许鹏[1] 陈长卿[2] 田磊[1] 李桐[2] 张冠军[1] 尹望[1] 陈光[1] 

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]吉林农业大学农学院,长春130118

出　　处：《农药》2013年第11期835-838,共4页Agrochemicals

基　　金：吉林农业大学博士后基金项目;吉林省自然科学基金项目(201115189);国家大学生创新创业训练计划(201210193004);吉林省博士后科研启动项目

摘　　要：[目的]菌株Bacillus methylotrophicus是一株对人参锈腐病菌(Cylindrocarpon destructans)有较强拮抗作用的细菌,为了有效防治人参锈腐病,进行了其产生抗菌蛋白的性质研究和抑菌效果试验。[方法]通过混菌法研究抗菌蛋白对紫外、热、酸碱、有机溶剂和蛋白酶的稳定性;采用生长速率法测定抗菌蛋白对锈腐病菌菌丝生长的抑制作用;平板对峙法测定其对锈腐病菌菌丝形态的影响;液培法测定其对锈腐病菌孢子萌发的抑制影响。[结果]抗菌蛋白具有很好的抗紫外线、耐热、耐酸碱和耐蛋白酶的稳定性,当质量浓度大于62.5 mg/L时,对人参锈腐病菌菌丝生长抑制率超过63.8%;显微镜观察其对菌丝生长有强烈的抑制作用,可以导致菌丝生长过程中分支增多、断裂和菌丝空洞,500 mg/L的抗菌蛋白对锈腐病菌孢子萌发的抑制率可达到100%。[结论]为人参锈腐病的防治提供新的生物防治资源。[Aims] Bacillus methylotrophicus has antagonistic action to Cylindrocarpon destructans. To prevent C. destructans of Panax ginseng, antagonistic protein and fungistasis experiments were carried out. [Methods] The stability of antagonistic protein to ultraviolet light, temperature, pH values, and protease were tested by mixing culture. The inhibition activity of antagonistic protein to C. destructans mycelium was tested by growth rate mensuration. The effect on mycelial morphology was tested by co-culture on plate and confrontation on slide. The effect on spore germination of C. destructans was tested by liquid culture. [Results] Antagonistic protein had a good stability to ultraviolet light, temperature, pH values and protease. The inhibition rate was 63.8% above against C. destructans＇ mycelium at more than 62.5 mg/L. Antagonistic protein had strong control effect on the growth of mycelium, which leads to increasing mycelium branches, rupture and mycelium cavity. At the concentration of 500 mg/L, its inhibition to spore germination reached up to 100%. [Conclusions] This study provides biological resources to control C. destructans of P. ginseng.

关 键 词：抗菌蛋白 性质 人参锈腐病菌 抑菌作用 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]