CMIA法与FPIA法检测环孢素A血药浓度的比较研究  

Comparison of Blood Concentration Monitoring of Cyclosporine A by CMIA and FPIA

作　　者：程丽静[1] 赵冠人[1] 冯端浩[1] 

机构地区：[1]解放军第309医院药剂科,北京100091

出　　处：《中国药房》2013年第42期3971-3973,共3页China Pharmacy

摘　　要：目的:比较化学发光微粒子免疫(CMIA)法与荧光偏振免疫(FPIA)法检测全血中环孢素A(CsA)浓度的相关性。方法:随机收集肾移植患者服药后的全血标本共50例和2013年3月国家卫生部室间质控血样标本,分别用CMIA法和FPIA法进行检测,考察结果的相关性及差异。结果:CMIA法和FPIA法检测结果的相关性方程为y=1.008x+48.561(r=0.950 0),结果存在显著性差异,FPIA法的测定结果大于CMIA。结论:两种方法检测CsA血药浓度同样具有可行性,但结果存在差异,因此临床调整用药时应注意监测方法的不同。OBJECTIVE： To compare the correlation of chemiluminescent microparticle immunoassay （CMIA） and fluores- cence polarization immunoassay （FPIA） for the determination of blood concentration of cyclosporine A （CsA）. METHODS： Whole blood samples of 50 patients underwent renal transplant and external quality control samples delivered by Ministry of Public Health in Mar. 2013 were randomly collected and determined by CMIA and FPLA respectively, The difference and correlation of 2 kinds of determination methods were investigated. RESULTS： The correlation of CMIA and FPIA was good and the regression equation was y=1.008x＋48.561 （r=0.950 0）, there was significant difference in results. CMIA provided significantly lower values than FPIA. CONCLUSIONS： Both methods are feasible for blood concentration monitoring of CsA but there are difference in determination re- sult. Great importance should be paid to CsA monitoring method because of the adjustment of medication method in the clinic.

关 键 词：环孢素A 荧光偏振免疫法 化学发光微粒子免疫法 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R979.5[医药卫生—药学]