艾灸调节急性胃溃疡大鼠胃黏膜细胞相关标志蛋白质表达的研究  

Regulation of moxibustion for expression of gastric mucosa cell-related marker protein in rats with acute gastric ulcer

作　　者：杨宗保[1] 王晨光[2] 龚安[2] 谢宇锋 刘琼[2] 杨青[2] 

机构地区：[1]厦门大学医学院中医系,福建厦门361005 [2]江西中医药大学 [3]深圳福田区中医院康复科

出　　处：《中国针灸》2013年第11期1017-1021,共5页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金项目:30960484;81260556;江西省自然科学基金项目:2008GZY 0022

摘　　要：目的:探讨艾灸促进胃黏膜损伤修复的相关物质基础。方法:将45只SD大鼠随机分为正常组、模型组、胃经穴组和对照点组,其中模型组15只,另3组每组10只。除正常组外,其余各组采用束缚-冷应激法制作胃黏膜损伤大鼠模型,胃经穴组和对照点组予以悬灸胃经穴"梁门""足三里"和对照点("梁门"、"足三里"两穴外侧旁开1cm处),每日1次,连续治疗12d。12d后光镜下观察胃黏膜形态学变化,蛋白质指纹图谱技术和弱阳离子、弱阴离子芯片检测大鼠胃黏膜细胞蛋白质指纹图谱,对比分析各组的差异蛋白质质/荷比峰。结果:与模型组比较,胃经穴组大鼠胃黏膜损伤指数值降低(P<0.05),胃黏膜形态学明显改善,与对照点组比较,胃经穴组大鼠胃黏膜损伤指数变化和形态学改善明显(P<0.05);弱阳离子芯片检测结果显示有4个标志蛋白质表达差异,艾灸胃经穴可调节分子量为1354Da、5692Da和8432Da 3个标志蛋白质表达升高(均P<0.05),3287Da标志蛋白质表达下降(P<0.05);弱阴离子芯片检测结果有3个标志蛋白质表达差异,艾灸胃经穴可调节分子量为2412Da、3026Da和6475Da 3个标志蛋白质表达升高(均P<0.05)。结论:艾灸胃经穴可调节急性胃溃疡大鼠胃黏膜细胞相关标志蛋白质差异表达,促进胃黏膜损伤的修复,效果优于穴位旁开1cm处。Objective To explore relevant material basis of moxibustion for recovering gastric mucosal lesion. Methods Forty-five SD rats were randomly divided into a normal group, a model group, an acupoint group and a control group, 15 rats in the model group and 10 rats in the rest three groups. Except the normal group, binding and cold stress method were used to establish gastric mucosa injury model. The suspended moxibustion was ap- plied in the acupoint group and control group at acupoints of the stomach meridian （＂Liangmen＂ （ST 21） and ＂Zusanli＂（ST 36）） and control acupoints （laterally l cm next to the ＂Liangmen＂ （ST 21） and ＂Zusanli＂ （ST 36））, once a day, consecutively for 12 days. After 12 days, morphology of gastric mucosal was observed under optical microscope; protein fingerprints of gastric mucosa cell in rats were detected by protein fingerprint technology, weak cation chip and weak anion chip. Also mass to charge ratio of differential proteins in groups were compared and analyzed. Results Compared with the model group, index of gastric mucosal lesion in the acupoint group was reduced and its morphology was obviously improved （P〈0.05）. Compared with the control group, in- dex and morphology of gastric mucosal lesion were significantly improved in the acupoint group （P〈 0. 05）. According to test of weak cation chip, there was four marker proteins that had expression differences, indicating moxibustion at acupoints of stomach meridian could increase expression of three marker protein whose molecular weight was 1354Da, 5692Da and 8432Da （all P〈0.05） while reduce expression of marker protein with molecular weight of 3287 Da （P〈0.05）. According to test of weak anion chip, moxibustion at acupoints of stomach meridian could increase expression of three marker proteins whose molecular weight was 2412 Da, 3026 Da and 6475 Da （all P〈0.05）. Conclusion Moxibustion at acupoints of the stomach meridian could regulate differential expression of gastric muco

关 键 词：急性胃溃疡 艾灸 蛋白质指纹图谱 

分 类 号：R245.8[医药卫生—针灸推拿学]