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作 者:郭柏福 朱滨[2] 万建义[1] 田家元[1] 舒德斌[1]
机构地区:[1]中国长江三峡集团公司中华鲟研究所,湖北宜昌443100 [2]水利部中国科学院水工程生态研究所,湖北武汉430079
出 处:《水产科学》2013年第10期573-578,共6页Fisheries Science
基 金:中国长江三峡集团公司中华鲟全人工繁殖研究项目(0799511)
摘 要:利用全自动生化分析仪,对人工淡水驯养3龄、8~10龄和14~16龄等3个年龄组的子一代中华鲟以及性腺发育至Ⅲ期的野生中华鲟共计四个类别中华鲟的蛋白质、血脂、血糖、血清酶类和无机离子等18项血液生化指标进行测定,以探究人工环境条件下子一代中华鲟的生物学特性。结果显示,随着人工养殖子一代中华鲟年龄的增加,血蛋白(血清总蛋白、白蛋白、球蛋白)和钠离子的含量增加,血脂(总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇)的含量呈“先降后升”趋势,血糖的含量呈“先升后降”趋势,血清酶类(谷草转氨酶、肌酸激酶、谷丙转氨酶、乳酸脱氢酶和碱性磷酸酶)的活性以3龄子一代中华鲟个体最高。野生Ⅲ期中华鲟有9项指标(氯离子、肌酸激酶、碱性磷酸酶、血清总蛋白、白蛋白、球蛋白、总胆固醇、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇)显著低于与其性腺发育状况相同的14~16龄人工养殖中华鲟( P<0.05),二者在其他9项血液生化指标间无显著性差异。研究表明,中华鲟的血液生化指标与其生理特点、生活环境、身体状况和性腺发育程度具有明显的相关性。Eighteen serum indices including protein ,fat ,glucose ,and inorganic ion levels and enzyme activity were monitored in 3 years old ,8~10 years old ,14~16 years old farmed F1 Chinese sturgeon (Acipenser sinensis) and the Chinese sturgeon with Ⅰ ,Ⅱ and Ⅲ stage gonads domesticated in artificial freshwater environment by automatic biochemical analyzer (type:7170A of Hitachi) to study the biological characteristics of Chinese sturgeon .The results showed that the levels of serum protein including total protein (TP) ,albumin(ALB) ,globulin (GLOB) and Na+ wereshown to be ascendant trend .The levels of serum fat including total cholesterol (CHOL) ,triglyceride (TRIG) ,high density lipoprotein cholesterol (HDLC) and low density lipoprotein cholesterol (LDLC ) were found to be decreased firstly ,and then increased as the time was elapsed while the concentration of serum glucose (GLU) was raised first and then decreased . The maximal activities of serum enzymes including glutamine-oxaloacetate (AST ) , creatine kinase (CK ) , glutamate-pyruvate transaminase (GPT ) , lactate dehydrogenase (LDH ) and alkaline phosphatase (ALP) were observed in the 3 year old individuals of F1 Chinese sturgeon .There were significantly lower 9 blood indices(Cl- ,CK ,ALP ,TP ,ALB ,GLOB ,CHOL ,HDLC and LDLC)in the wild Chinese sturgeon than in 14 ~ 16 years old group of farmed F1 Chinese sturgeon with same developmental gonads (P0 .05 ) . T he findings indicate that the significant differences in blood biochemistry characteristics are involved in the inherent background variability of these sturgeon individuals ,including exposure history in different habitats ,different developmental gonad and physiological characteristics .
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