毛脉酸模内生真菌RGT-S4生长曲线及菌丝体化学成分初步研究  

Growth Curve and Chemical Constituents of RGT-S4 Mycelium from Rumex Gmelini Turcz

作　　者：王星星[1,2] 王振月[1] 王博[1,3] 卫丽曼[1] 郑成琳[1] 

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]江苏康缘药业股份有限公司现代中药研究院妇科新药研究所,江苏连云港222001 [3]黑龙江珍宝岛药业药物研究院,黑龙江哈尔滨150000

出　　处：《中医药学报》2013年第5期17-19,共3页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(No.30970300);哈尔滨市科技创新人才研究专项资金项目(优秀学科带头人)(No.2010RFXXS031)

摘　　要：目的:研究毛脉酸模(Rumex gmelini Turcz.)内生真菌RGT-S4的生长曲线及菌丝体中的化学成分。方法:运用液体培养法对毛脉酸模内生真菌RGT-S4的生长曲线进行研究,同时运用溶剂萃取、硅胶柱色谱、ODS反相柱色谱、Sephadex LH-20柱色谱、制备液相等方法对其醇提物正丁醇部位进行分离纯化,根据理化性质、NMR数据鉴定化合物的结构。结果:最佳生长天数为7天;同时分离到3个化合物,其结构鉴定为:甘露醇(Ⅰ)、D-果糖(Ⅱ)、L-2-O-甲基-手-肌醇(Ⅲ)。结论:对其生长天数进行研究,以优化其培养条件,同时以上化学成分均为首次从毛脉酸模内生真菌RGT-S4菌丝体中分离得到。Objective：To study the growth curve and chemical constituents of RGT-S4 mycelium from Rumex gmelini Turcz..Method：We studied growth curve by using liquid cultivation,and ethanol extracts by using n-BuOH extraction,solvent extraction,silica gel column chromatography,ODS column chromatography,Sephadex LH-20 and other separation means.The structures were identified according to physical and chemical properties and date of NMR.Results：The best growth days were seven days,and three compounds were identified as Mannitol（Ⅰ）,D-fructose（Ⅱ）,L-2-O-methyl-chiro-inositol（Ⅲ）.Conclusion：Growth days are studied in order to optimizate the culture conditions,and the above compounds are identified from RGT-S4 mycelium of Rumex gmelini Turcz.for the frist time.

关 键 词：毛脉酸模 内生真菌 曲霉属 生长曲线 化学成分 

分 类 号：R284.2[医药卫生—中药学]