进境棉籽中胶孢炭疽病菌的分离与鉴定  

Isolation and Identification of Colletotrichum gloeosporioides in Imported Cottonseed

作　　者：孙娟[1] 陈舜胜[1] 于子翔[2] 于翠[2] 杨翠云[2] 

机构地区：[1]上海海洋大学,食品科学与工程学院,上海201306 [2]上海出入境检验检疫局,上海200135

出　　处：《上海交通大学学报（农业科学版）》2013年第5期7-11,15,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海检验检疫局科技项目(HK010-2012);上海市科委技术标准专项(11dz0502700)

摘　　要：对一批澳大利亚进境的棉籽进行了病原菌分离,在棉籽上分离到1株疑似胶孢炭疽病菌的菌株CG-92963。通过对该菌株进行病原菌形态学观察、致病性测定并结合分子生物学方法检测。结果表明,菌株CG-92963在PDA上产生大量分生孢子,分生孢子呈长椭圆形或圆筒形;分别用真菌通用引物、特异引物对CG-92963进行扩增和测序,Blast分析结果表明其与GenBank中胶孢炭疽病菌Colletotrichum gloeosporioides序列同源性为100%;该菌不仅侵染棉花,还侵染芒果和梨。根据上述实验结果,将分离获得的菌株CG-92963鉴定为胶孢炭疽病菌Colletotrichum gloeosporioides(Penz)Sacc。The suspected strain CG-92963 of Colletotrichum gloeosporioides was isolated in imported cottonseed from Auatralia in Shanghai port by traditional PDA method. The morphological characteristics, molecular and pathogenicity of CG-92963 were tested. The results showed that CG-92963 produced massive conidia on PDA, the conidia is oval or cylindrical. Universal primers and special primers were used to amplify and sequence from CG-92963. Compared with Colletotrichum gloeosporioides from GenBank databases by blast method, the homology was 100%. Pathogenicity tests showed that Gossypium Linn, Mangifera indica Linn and Pyrus bretschneideri Rehd were infected by CG-92963. The isolate CG-92963 was identified as Colletotrichum gloeosporioides （Penz.）Sacc.

关 键 词：棉籽 胶孢炭疽病菌 分离与鉴定 

分 类 号：S435.621.22[农业科学—农业昆虫与害虫防治]