右美托咪定预处理对大鼠心肌缺血再灌注损伤的保护作用及补体3蛋白的影响  

The protective effect of dexmedetomidine preconditioning and its effect on complement component 3 in rats with myocardial ischemia reperfusion injury

作　　者：莫晓飞[1] 陈刘芳[1] 万晶[1] 吴云[1] 柯剑娟[1] 

机构地区：[1]武汉大学中南医院麻醉科,湖北武汉430071

出　　处：《中国药物应用与监测》2013年第5期258-260,共3页Chinese Journal of Drug Application and Monitoring

基　　金：国家自然科学基金(81201499);湖北省自然科学基金(2010CDB00403)

摘　　要：目的:探讨右美托咪定预处理对大鼠心肌缺血再灌注损伤的保护作用及对补体3(C3)蛋白的影响。方法:SD雄性大鼠45只,随机分为3组(n=15):假手术组(Sham组)、缺血再灌注组(I/R组)、右美托咪定预处理组(DEX组)。DEX组自缺血前2 h持续静脉输注右美托咪定5 mL·kg-1(用0.9%氯化钠注射液将右美托咪定稀释为1μg·mL-1)直到结扎心脏冠状动脉左前降支前停止,输注时间为2 h;Sham组及I/R组在相同的时间内按照5 mL·kg-1速率输注等量0.9%氯化钠注射液。再灌注120 min时,取心脏组织,测定心肌梗死面积,用蛋白质印迹法(Western blot)测定心肌组织中C3蛋白的表达。结果:与Sham组比较,I/R组和DEX组的心肌梗死面积、C3蛋白表达明显升高(P<0.05);与I/R组比较,DEX组的心肌梗死面积和C3蛋白表达明显降低(P<0.05)。结论:右美托咪定预处理能够减轻大鼠心肌缺血再灌注损伤,其机制与减少补体3蛋白表达有关。Objective： To investigate the protective effect and possible mechanism （effect on complement component 3） of dexmedetomidine preconditioning on the myocardium of ischemia and reperfused rats. Methods： Forty-five Sprague-Dawley male rats were divided into sham operation group（ Sham group ）, ischemic-reperfusion group （I/R group） and dexmedetomidine preconditioning group （DEX group） randomly, with 15 rats in each group. Before left anterior descending coronary artery was clamped, DEX group received dexmedetomidine with 5 mL·kg^-1 body weight for 2 h （dexmedetomidine was diluted to 1 μg·mL^-1 with 0.9% sodium chloride injection）. While FR group and Sham group were infused 0.9% sodium chloride injection with 5 mL·kg^-1 body weight for 2 h. At the end of 120 min reperfusion, the myocardial tissues were taken for determination of the infarct size and C3 expression using the Western blot method. Results： Compared with the Sham group, the infarct sizes and expressions of C3 increased obviously in the lfR group and DEX group （P 〈 0.05）. Compared with the I/R group, the infarct sizes in DEX group were smaller and expressions of C3 were lower（ P 〈 0.05）. Conclusion： Dexmedetomidine could attenuate myocardial ischemia- reperfusion injury in rats via suppressing the expression of C3.

关 键 词：缺血再灌注 补体3 右美托咪定 

分 类 号：R965[医药卫生—药理学] R614[医药卫生—药学]