评价血清中结核杆菌特异性蛋白抗体对诊断肺结核的应用价值  

Clinical significance of serum tuberculosis specific antigen (TB-SA) antibody in the diagnosis of pulmonary tuberculosis

作　　者：张慧慧[1] 熊国亮[1] 

机构地区：[1]江西省胸科医院,江西南昌330006

出　　处：《实验与检验医学》2013年第5期419-420,430,共3页Experimental and Laboratory Medicine

基　　金：江西省卫生厅科技资助项目(编号:20053180)

摘　　要：目的评价结核分枝杆菌特异性蛋白抗体检测对肺结核诊断的应用价值。方法对370例活动性肺结核患者、42例非结核性肺部疾病患者和30例健康献血员进行血清结核分枝杆菌特异性蛋白抗体检测,同时对全部肺结核和非结核性肺部疾病患者进行痰抗酸杆菌涂片和培养。结果 370例临床诊断的活动性肺结核患者,结核分枝杆菌特异性蛋白抗体检测阳性284例,阳性率76.8%;42例非结核性肺部疾病患者,假阳性4例,假阳性率9.5%;30例健康献血员对照组,假阳性1例,假阳性率3.3%。检测菌阳肺结核的敏感性为81.4%,,菌阴肺结核的敏感性为72.9%;阳性检出率76.8%远高于细菌学的阳性检出率45.1%,χ2=77.72,P<0.05,具有统计学意义。结论结核分枝杆菌特异性蛋白抗体检测对肺结核的诊断具有较好的敏感性和特异性。Objective To evaluate the significance of detecting serum tuberculosis specific antigen （TB-SA） antibody in the diagnosis of pulmonary tuberculosis. Methods TB-SA antibody in the serum samples from 370 cases of active pulmonary tuberculosis, 42 cases with non-tuberculosis pulmonary disease and 30 healthy volunteers were detected using enzyme-linked immunosor- bent assay （ELISA）. Results Two hundred and eighty-four out of 370 active pulmonary tuberculosis patients had positive results of TB-SA antibody tested by using ELISA, the positive rate was 76.8%, 4 of 42 patients with other pulmonary diseases and 1 of 30 healthy people had false positive results ,the false postive rates were 9.5% and 3.3%, respectively. The sensitivity of TB-SA anti- body in diagnosis of bacteriological positive and negative pulmonary tuberculosis patients were 81.4% and 72.9%,respectively. With a total sensitivity of 76.8%,the positive rate of TB-SA detection in the diagnosis of tuberculosis was 76.8%,which was far higher than that of 45.1% by using bacteriological examination. The difference between two methods had statistical significance （P〈 0.05）. Conclusion Detection of Mycobacterium tuberculosis specific antibody has better sensitivity and specificity in diagnosis of tuberculosis

关 键 词：结核分枝杆菌 抗体 诊断 

分 类 号：R446.62[医药卫生—诊断学] R521[医药卫生—临床医学]