PCR-SSP法检测初筛RhD阴性样本的基因研究  

Genetic Research on Detection of Screening RhD Negative Samples by PCR-SSP Method

作　　者：陈冬 

机构地区：[1]西安市中心血站检验科,西安710061

出　　处：《现代检验医学杂志》2013年第5期45-47,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 通过研究西安地区血清抗-D阳性样本的基因,确定D抗原有免疫反应的样本多样性.方法 采用PCR-SSP的方法对初筛RhD阴性的无偿献血者和临床样本血清抗-D阳性样本进行基因分型,总结血清中产生抗-D的样本的基因多样性.结果 在47例样本中,检出d/d基因型40例,弱D15型2例,RhD-CE（2-9）-D/d融合基因型3例,DⅥⅢ型1例和RhD710delC型1例.结论 D抗原的缺失和改变都会导致D抗原的免疫应答.Objective To study the anti-D positive serum samples of Xi＇an region gene and determine D antigen immune re-sponse sample diversity. Methods PCR-SSP method for screening RhD negative blood donors and clinical samples of serum anti-D positive samples were genotyped, summarized the production of anti-D in serum samples of gene diversity. Results In the 47 cases, detection of d/d gene type were 40 eases,2 cases of weak type D15 ,RhD-CE （2-9）-D/d fusion gene type in 3 cases,1 cases of D type Ⅲ and 1 cases of type RhD710delC. Conclusion The changes of D antigen and lack of will lead to immune response of D antigen.

关 键 词：RhD阴性的无偿献血者 临床样本 RH阴性孕妇 D抗原同种免疫反应 DEL型 

分 类 号：Q503[生物学—生物化学] R457.12[医药卫生—治疗学]