荧光光谱法研究淫羊藿素与牛血清白蛋白的相互作用  

Fluorescence spectrometry for interaction of icaritin and bovine serum albumin

作　　者：林坚涛[1] 李颖[1] 梁冬丽[1] 尤婷婷[1] 潘文嘉[1] 崔红晶[1] 吴铁[1] 

机构地区：[1]广东医学院中药与新药研究所,广东东莞523808

出　　处：《黑龙江医药科学》2013年第5期11-12,共2页Heilongjiang Medicine and Pharmacy

基　　金：广东省科技计划项目;编号:2011B031800248;广东医学院2011年面上项目;编号:XK1203

摘　　要：目的:考察淫羊藿素与牛血清白蛋白(BSA)的相互作用机制。方法:利用荧光光谱考察了两者之间的荧光淬灭类型,计算结合数(n)和结合常数。结果:淫羊藿素与BSA作用可导致BSA内源荧光猝灭,采用Stern-Volmer拟合方程和Line-Weaver-Burk拟合方程,发现其猝灭机理为静态猝灭,结合常数Ka=4.17×1011 L/mol,结合位点数n=2.63。结论:淫羊藿素可与牛血清白蛋白发生结合形成复合物,并使牛血清蛋白产生静态淬灭。Objective：To study the interaction of Icaritin with bovine serum albumin. Methods：We adopted fluorescence spectrometry to observe the types of fluorescence quenching of both Icaritin and bovine serum albumin and calculated the binding number and binding constant. Results：The results showed that these interactions resulted in the endogenous fluorescence quenching of bovine serum albumin, which belonged to a static quenching mechanism. The binding constant Ka was 4.17×1011L/mol and the Binding Site Number was 2.63. Conclusion：Icaritin could form complexes by combining bovine serum albumin and make bovine serum albumin produce static quenching.

关 键 词：淫羊藿素 牛血清白蛋白 荧光光谱 

分 类 号：R284[医药卫生—中药学]