LPS诱导人肾小球系膜细胞FoxM1B分子表达的研究  

FoxM1B molecular expression of human glomerular mesangial cells induced by LPS

作　　者：陈伟强[1] 魏凤香[2] 潘德顺[3] 辛华[4] 王莉[4] 王琳[4] 

机构地区：[1]广东药学院基础学院,广东广州510006 [2]深圳市龙岗区妇幼保健院中心实验室,广东深圳518000 [3]广东药学院药科学院,广东广州510006 [4]佳木斯大学附属第一医院,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2013年第5期20-21,共2页Heilongjiang Medicine and Pharmacy

基　　金：广东省卫生厅项目;编号:A2010285

摘　　要：目的:探讨脂多糖(LPS)对系膜细胞FoxM1B表达的影响。方法:体外培养系膜细胞,分别用LPS(10Pg/mI)处理12、24、48h,利用MTT检测系膜细胞增殖、Real-Time PCR及Western blot观察细胞FoxM1BmRNA及蛋白表达水平。结果:与正常对照组相比,在12、24、48h时间段,10Pg/mL的LPS能明显促进人肾小球系膜细胞增殖;静息状态的系膜细胞表达FoxM1BmRNA及蛋白极其微量,LPS(10pg/mL)可诱导系膜细胞显著表达FoxM1B。结论:LPS诱导人肾小球系膜细胞增生的机制可能与FoxM1B表达增加相关。Objective： To investigate the influence of LPS on FoxM1B molecular expression of human glomerular mesangial cells. Methods： Cultured glomerular mesangial cells were exposed to LPS for 12,24,48 hours. MTT were used to check the cells growths, Real--Time PCR were used to observe FoxM1B mRNA. The protein expression of FoxM1B was determined by Western blot. Resul ts.Compared with the control group,glomerular mesangial cells proliferation of LPS group （10 Pg /mI）significantly increased at 12,24,48 hours. The expression of FoxM1B mRNA and the level of FoxMIB protein were significantly increased in mesangial cells under LPS medium at 12.24.48 hours. Conclusion：The mechanism of LPS stimulates glomerular mesangial cells proliferation may be relative to the increase of FoxM1B expression.

关 键 词：系膜细胞 脂多糖 FoxM1B基因 

分 类 号：Q343[生物学—遗传学]