切花菊‘南农银山’与紫花野菊种间杂交及后代耐涝性鉴定  

Interspecific Hybridization Between Chrysanthemum morifolium ‘Nannongyinshan’ and C.zawadskii and Identification of Waterlogging Tolerance of Their Hybrid

作　　者：王靓[1] 王楚楚[1] 蒋甲福[1] 陈素梅[1] 房伟民[1] 滕年军[1] 管志勇[1] 廖园[1] 陈发棣[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《中国农业科学》2013年第20期4328-4335,共8页Scientia Agricultura Sinica

基　　金：江苏省科技支撑计划(BE2011325;BE2012350);国家"948"项目(2013-S13);江苏省农业科技自主创新资金项目(CX(12)2020);教育部新世纪优秀人才支持计划(NCET-10-0492);国家自然科学基金(31272196;31171983)

摘　　要：【目的】对切花小菊‘南农银山’和紫花野菊进行种间杂交,并对后代的表型和耐涝性进行鉴定,以期对切花小菊的耐涝性进行改良。【方法】以切花小菊‘南农银山’为母本(不耐涝)、紫花野菊为父本(耐涝)开展人工杂交。杂交后代通过形态学和SRAP分子标记进行鉴定。耐涝性通过测定淹水处理后叶片相对含水量、叶绿素含量、丙二醛含量和抗氧化酶活性进行鉴定。【结果】获得1个种间杂交后代,其株高、叶长、叶宽、管状花数、舌状花数等形态指标均介于双亲之间;SRAP分子标记分析发现,子代中同时具有父、母本特异条带,说明获得的后代是真杂种。淹水处理后,子代的叶片相对含水量、叶绿素含量的降低、丙二醛含量的升高显著低于母本‘南农银山’,且具有比母本更高的抗氧化酶活性,表现出较强的耐涝性。【结论】通过种间杂交能将紫花野菊的耐涝性导入切花小菊品种,进行切花小菊种质耐涝性改良和创新。[ Objective ] To improve the waterlogging tolerance of spray cut chrysanthemum, an interspecific hybridization was combined between Chrysanthemum morifolium ＇Nannongyinshan＇ and C. zawadskii. The phenotypic characteristics and waterlogging tolerance of the parents and the hybrid were identified. [Method] Interspecific hybridization was made between the female parent C. morifolium ＇Nannongyinshan＇, which is susceptible to waterlogging ,and the male parent C. zawadskii, which is tolerant. The hybridity was identified by morphological traits and SRAP molecular markers. Watedogging tolerance was investigated by changes in leaf relative water content （RWC）, contents of chlorophyll （Chl） and malondialdehyde （MDA）, and activities of antioxidant enzymes. [Result] Only one putative hybrid was obtained through the interspecific hybridization. Morphological characteristics including the plant height, leaf length, leaf width, number of tubular florets and ray florets of the hybrid were different with that of the parents. In the hybrid, both the male and the female specific bands were observed by SRAP molecular markers analysis. Thus the progeny was a real interspecific hybrid. The decrease of RWC and chl and the increase of MDA of the hybrid were significantly lower than that of maternal ＇Nannongyinshan＇, while the activities of antioxidant enzymes were higher. [ Conclusion ] The results indicated that the hybrid was more tolerant to waterlogging than ＇Nannongyinshan＇. Therefore, it is possible to obtain new waterlogging tolerant cut chrysanthemum germplasm via interspecific hybridization to introduce the tolerant trait of C. zawadskii.

关 键 词：菊花 紫花野菊 种间杂交 耐涝性 

分 类 号：S682.11[农业科学—观赏园艺]