植物硫激素PSK-α在水稻成熟胚培养中的作用  

Effects of Plant Phytosulfokine PSK-α on Rice Mature Embryo Culture

作　　者：陈德西[1] 何忠全[1] 李仕贵[2] 向运佳[1] 

机构地区：[1]四川省农业科学院植物保护研究所,四川成都610066 [2]四川农业大学水稻研究所,四川成都611130

出　　处：《西南农业学报》2013年第5期1753-1756,共4页Southwest China Journal of Agricultural Sciences

基　　金：四川省财政基因工程优秀论文基金项目(2011LWJJ-005);农业部西南作物有害生物综合治理重点实验室

摘　　要：本文对不同浓度的PSK-α在水稻成熟胚的愈伤诱导、愈伤继代和分化过程中效应进行了研究。结果表明,在成熟胚诱导效率方面,N6和NB培养基优于MS和CC培养基;在同一培养基上,不同浓度的PSK-α对成熟胚的诱导效率不同;同一浓度的PSK-α对不同品种的成熟胚诱导效应不同。明恢63在50 pmol/L PSK-α的MS培养基上、CDR22在300 pmol/L PSK-α的N6培养基上、蜀恢527在100 pmol/L PSK-α的NB培养基上、地谷在100 pmol/L PSK的N6培养基上成熟胚诱导效率较好,分别达到24%、79.0%、85.4%和67.9%。PSK-α对愈伤的继代没有促进作用,但易诱导愈伤生根。在分化过程中,添加50 pmol/L PSK-α能使愈伤现绿早、愈伤状态好,分化率提高近一倍。In this study,PSK-α function in mature embryo callus induction,callus subculture and differentiation progress was studied.The results showed that N6 and NB medium for mature embryos induction efficiency were better than MS and CC medium.In the same medium,different concentrations PSK-α in induction medium had different induction efficiency for mature embryo.Even the same concentration PSK-αin induction medium also had different induction efficiency for mature embryo in different rice materials.The better efficiencies of mature embryo of Minghui 63 in the MS medium with 50 pmol/LPSK-α,CDR22 in N6 medium with 300 pmoL/L PSK-α,Shuhui 527 in NB medium with 100 pmol/L PSK-α and Digu in the N6 medium with 100 pmol/L PSK-α,were 24 ％,79.0 ％,85.4 ％ and 67.9 ％,respectively.PSK had no promotion function in callus subculture process,but it easily induced rooting callus.In the differentiation process,50 pmol/L PSK-α could promote the callus keeping good status and early differentiated,and their differentiation rate nearly was higher 100 ％ than that of control.

关 键 词：植物硫激素PSK-α 水稻 成熟胚 诱导 分化 

分 类 号：S511.035[农业科学—作物学]